TGF-β1 modifications in nuclear matrix proteins of osteoblasts during differentiation

Danielle Lindenmuth, André J. Van Wijnen, Sheldon Penman, Janet L. Stein, Gary S. Stein, Jane B. Lian

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Nuclear matrix protein (NMP) composition of osteoblasts shows distinct two-dimensional gel electrophoretic profiles of labeled proteins as a function of stages of cellular differentiation. Because NMPs are involved in the control of gene expression, we examined modifications in the representation of NMPs induced by TGF-β1 treatment of osteoblasts to gain insight into the effects of TGF-β on development of the osteoblast phenotype. Exposure of proliferating fetal rat calvarial derived primary cells in culture to TGF-β1 for 48 h (day 4-6) modifies osteoblast cell morphology and proliferation and blocks subsequent formation of mineralized nodules. Nuclear matrix protein profiles were very similar between control and TGF-β-treated cultures until day 14, but subsequently differences in nuclear matrix proteins were apparent in TGF-β-treated cultures. These findings support the concept that TGF-β1 modifies the final stage of osteoblast mineralization and alters the composition of the osteoblast nuclear matrix as reflected by selective and TGF-β-dependent modifications in the levels of specific nuclear matrix proteins. The specific changes induced by TGF-β in nuclear matrix associated proteins may reflect specialized mechanisms by which TGF-β signalling mediates the alterations in cell organization and nodule formation and/or the consequential block in extracellular mineralization.

Original languageEnglish (US)
Pages (from-to)291-303
Number of pages13
JournalJournal of cellular biochemistry
Volume69
Issue number3
DOIs
StatePublished - Jun 1 1998

Keywords

  • Bone
  • Mineralization
  • Nuclear matrix
  • Osteoblast differentiation
  • TGF-β1

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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