TGFβ-inducible early gene (TIEG) also codes for early growth response a (EGRα): Evidence of multiple transcripts from alternate promoters

Michael P Fautsch, Anne Vrabel, Malayannan Subramaniam, Theresa E. Hefferen, Thomas C. Spelsberg, Eric D Wieben

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

TGFβ-inducible early gene (TIEG) and early growth response α (EGRα) are putative transcription factors based on homology to known zinc finger proteins SP1, EGR1, BTEB, and Wilm tumor. Here we report that TIEG and EGRα are expressed from alternative promoters of the same gene. The TIEG/EGRα gene spans 8 kb and contains five exons. Use of alternative first exons results in TIEG having 12 unique amino acids on its N-terminus. Computer analysis of the 5' upstream regions of either TIEG (exon 1a) or EGRα (exon 1b) does not identify a TATA box or initiator sequence but shows consensus sequence similarities to binding sites for several transcription factors including SP1, JunB, and aromatic hydrocarbon/receptor-ligand complexes. Analysis of constructs containing 5'-flanking regions show that both the TIEG and the EGRα promoters have significant activity in human fetal osteoblast cells. Northern analysis of mRNA from various human tissues and several cell lines reveals that TIEG is the predominant transcript produced and regulated by growth factors from the TIEG/EGRα gene.

Original languageEnglish (US)
Pages (from-to)408-416
Number of pages9
JournalGenomics
Volume51
Issue number3
DOIs
StatePublished - Aug 1 1998

Fingerprint

Growth
Genes
Exons
Early Growth Response Transcription Factors
Aromatic Hydrocarbons
TATA Box
5' Flanking Region
Zinc Fingers
Consensus Sequence
Osteoblasts
Human Activities
Intercellular Signaling Peptides and Proteins
Transcription Factors
Binding Sites
Ligands
Amino Acids
Cell Line
Messenger RNA
Neoplasms
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

TGFβ-inducible early gene (TIEG) also codes for early growth response a (EGRα) : Evidence of multiple transcripts from alternate promoters. / Fautsch, Michael P; Vrabel, Anne; Subramaniam, Malayannan; Hefferen, Theresa E.; Spelsberg, Thomas C.; Wieben, Eric D.

In: Genomics, Vol. 51, No. 3, 01.08.1998, p. 408-416.

Research output: Contribution to journalArticle

Fautsch, Michael P ; Vrabel, Anne ; Subramaniam, Malayannan ; Hefferen, Theresa E. ; Spelsberg, Thomas C. ; Wieben, Eric D. / TGFβ-inducible early gene (TIEG) also codes for early growth response a (EGRα) : Evidence of multiple transcripts from alternate promoters. In: Genomics. 1998 ; Vol. 51, No. 3. pp. 408-416.
@article{7973fd60f27246b7aa6818d128bf9bdf,
title = "TGFβ-inducible early gene (TIEG) also codes for early growth response a (EGRα): Evidence of multiple transcripts from alternate promoters",
abstract = "TGFβ-inducible early gene (TIEG) and early growth response α (EGRα) are putative transcription factors based on homology to known zinc finger proteins SP1, EGR1, BTEB, and Wilm tumor. Here we report that TIEG and EGRα are expressed from alternative promoters of the same gene. The TIEG/EGRα gene spans 8 kb and contains five exons. Use of alternative first exons results in TIEG having 12 unique amino acids on its N-terminus. Computer analysis of the 5' upstream regions of either TIEG (exon 1a) or EGRα (exon 1b) does not identify a TATA box or initiator sequence but shows consensus sequence similarities to binding sites for several transcription factors including SP1, JunB, and aromatic hydrocarbon/receptor-ligand complexes. Analysis of constructs containing 5'-flanking regions show that both the TIEG and the EGRα promoters have significant activity in human fetal osteoblast cells. Northern analysis of mRNA from various human tissues and several cell lines reveals that TIEG is the predominant transcript produced and regulated by growth factors from the TIEG/EGRα gene.",
author = "Fautsch, {Michael P} and Anne Vrabel and Malayannan Subramaniam and Hefferen, {Theresa E.} and Spelsberg, {Thomas C.} and Wieben, {Eric D}",
year = "1998",
month = "8",
day = "1",
doi = "10.1006/geno.1998.5388",
language = "English (US)",
volume = "51",
pages = "408--416",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - TGFβ-inducible early gene (TIEG) also codes for early growth response a (EGRα)

T2 - Evidence of multiple transcripts from alternate promoters

AU - Fautsch, Michael P

AU - Vrabel, Anne

AU - Subramaniam, Malayannan

AU - Hefferen, Theresa E.

AU - Spelsberg, Thomas C.

AU - Wieben, Eric D

PY - 1998/8/1

Y1 - 1998/8/1

N2 - TGFβ-inducible early gene (TIEG) and early growth response α (EGRα) are putative transcription factors based on homology to known zinc finger proteins SP1, EGR1, BTEB, and Wilm tumor. Here we report that TIEG and EGRα are expressed from alternative promoters of the same gene. The TIEG/EGRα gene spans 8 kb and contains five exons. Use of alternative first exons results in TIEG having 12 unique amino acids on its N-terminus. Computer analysis of the 5' upstream regions of either TIEG (exon 1a) or EGRα (exon 1b) does not identify a TATA box or initiator sequence but shows consensus sequence similarities to binding sites for several transcription factors including SP1, JunB, and aromatic hydrocarbon/receptor-ligand complexes. Analysis of constructs containing 5'-flanking regions show that both the TIEG and the EGRα promoters have significant activity in human fetal osteoblast cells. Northern analysis of mRNA from various human tissues and several cell lines reveals that TIEG is the predominant transcript produced and regulated by growth factors from the TIEG/EGRα gene.

AB - TGFβ-inducible early gene (TIEG) and early growth response α (EGRα) are putative transcription factors based on homology to known zinc finger proteins SP1, EGR1, BTEB, and Wilm tumor. Here we report that TIEG and EGRα are expressed from alternative promoters of the same gene. The TIEG/EGRα gene spans 8 kb and contains five exons. Use of alternative first exons results in TIEG having 12 unique amino acids on its N-terminus. Computer analysis of the 5' upstream regions of either TIEG (exon 1a) or EGRα (exon 1b) does not identify a TATA box or initiator sequence but shows consensus sequence similarities to binding sites for several transcription factors including SP1, JunB, and aromatic hydrocarbon/receptor-ligand complexes. Analysis of constructs containing 5'-flanking regions show that both the TIEG and the EGRα promoters have significant activity in human fetal osteoblast cells. Northern analysis of mRNA from various human tissues and several cell lines reveals that TIEG is the predominant transcript produced and regulated by growth factors from the TIEG/EGRα gene.

UR - http://www.scopus.com/inward/record.url?scp=0032129209&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032129209&partnerID=8YFLogxK

U2 - 10.1006/geno.1998.5388

DO - 10.1006/geno.1998.5388

M3 - Article

C2 - 9721211

AN - SCOPUS:0032129209

VL - 51

SP - 408

EP - 416

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 3

ER -