TGFβ inducible early gene enhances TGFβ/Smad-dependent transcriptional responses

Steven A. Johnsen, Malayannan Subramaniam, Ralf Janknecht, Thomas C. Spelsberg

Research output: Contribution to journalArticle

114 Scopus citations

Abstract

TGFβ inducible early gene (TIEG) encodes a three zinc-finger Krüppel-like transcription factor whose overexpression has been shown to mimic the effects of TGFβ in human osteosarcoma and pancreatic carcinoma cells. In order to investigate a potential role of TIEG in the TGFβ signal transduction pathway, we studied its impact on a Smad binding element (SBE) reporter which is known to be regulated by TGFβ through the R-Smad proteins. We demonstrate that TIEG overexpression enhances TGFβ induction of SBE reporter activity. TIEG overexpression also enhances induction of the endogenous TGFβ regulated genes p21 and PAI-1. The ability of TIEG to enhance TGFβ actions is Smad dependent since TIEG has no effect on SBE transcription in the absence of Smad4 expression or when an inhibitory Smad protein, Smad7, is overexpressed. Furthermore, TIEG overexpression enhances TGFβ induced Smad2 phosphorylation. Lastly, TIEG appears to function by binding to and thereby repressing a specific element in the proximal promoter of the inhibitory Smad7 gene. In conclusion, these results describe a novel mechanism for the potentiation of TGFβ/Smad signaling via repression of the inhibitory Smad7 gene by TIEG.

Original languageEnglish (US)
Pages (from-to)5783-5790
Number of pages8
JournalOncogene
Volume21
Issue number37
DOIs
StatePublished - Aug 22 2002

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Keywords

  • Smad
  • TGFβ
  • TIEG
  • Transcription
  • Zinc-finger

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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