Temperature dependence of muscarinic acetylcholine receptor activation, desensitization, and resensitization

E. El-Fakahany, E. Richelson

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

The effects of temperature on muscarinic acetylcholine receptor activation, densitization, and resensitization were studied with the use of intact mouse neuroblastoma cells (clone N1E-115), which have muscarinic receptors that mediate cyclic GMP synthesis. Below 15-20°C, activation or desensitization of muscarinic receptors by carbamylcholine and recovery from desensitization (caused by carbamylcholine at 37°C) did not occur. Above these temperatures, the apparent rates of receptor-mediated cyclic GMP synthesis, desensitization, and recovery of sensitivity increased at the incubation temperature was increased. Arrhenius plots of the data yielded activation energies of 25, 14, and 23 kcal.mol-1 for activation, desensitization, and resensitization, respectively. These data suggest that a certain degree of membrane phospholipid fluidity is required for these processes to occur.

Original languageEnglish (US)
Pages (from-to)1288-1295
Number of pages8
JournalJournal of Neurochemistry
Volume34
Issue number5
StatePublished - 1980

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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