Abstract
The effects of temperature on muscarinic acetylcholine receptor activation, densitization, and resensitization were studied with the use of intact mouse neuroblastoma cells (clone N1E-115), which have muscarinic receptors that mediate cyclic GMP synthesis. Below 15-20°C, activation or desensitization of muscarinic receptors by carbamylcholine and recovery from desensitization (caused by carbamylcholine at 37°C) did not occur. Above these temperatures, the apparent rates of receptor-mediated cyclic GMP synthesis, desensitization, and recovery of sensitivity increased at the incubation temperature was increased. Arrhenius plots of the data yielded activation energies of 25, 14, and 23 kcal.mol-1 for activation, desensitization, and resensitization, respectively. These data suggest that a certain degree of membrane phospholipid fluidity is required for these processes to occur.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1288-1295 |
| Number of pages | 8 |
| Journal | Journal of Neurochemistry |
| Volume | 34 |
| Issue number | 5 |
| State | Published - 1980 |
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ASJC Scopus subject areas
- Biochemistry
- Cellular and Molecular Neuroscience
Cite this
Temperature dependence of muscarinic acetylcholine receptor activation, desensitization, and resensitization. / El-Fakahany, E.; Richelson, E.
In: Journal of Neurochemistry, Vol. 34, No. 5, 1980, p. 1288-1295.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Temperature dependence of muscarinic acetylcholine receptor activation, desensitization, and resensitization
AU - El-Fakahany, E.
AU - Richelson, E.
PY - 1980
Y1 - 1980
N2 - The effects of temperature on muscarinic acetylcholine receptor activation, densitization, and resensitization were studied with the use of intact mouse neuroblastoma cells (clone N1E-115), which have muscarinic receptors that mediate cyclic GMP synthesis. Below 15-20°C, activation or desensitization of muscarinic receptors by carbamylcholine and recovery from desensitization (caused by carbamylcholine at 37°C) did not occur. Above these temperatures, the apparent rates of receptor-mediated cyclic GMP synthesis, desensitization, and recovery of sensitivity increased at the incubation temperature was increased. Arrhenius plots of the data yielded activation energies of 25, 14, and 23 kcal.mol-1 for activation, desensitization, and resensitization, respectively. These data suggest that a certain degree of membrane phospholipid fluidity is required for these processes to occur.
AB - The effects of temperature on muscarinic acetylcholine receptor activation, densitization, and resensitization were studied with the use of intact mouse neuroblastoma cells (clone N1E-115), which have muscarinic receptors that mediate cyclic GMP synthesis. Below 15-20°C, activation or desensitization of muscarinic receptors by carbamylcholine and recovery from desensitization (caused by carbamylcholine at 37°C) did not occur. Above these temperatures, the apparent rates of receptor-mediated cyclic GMP synthesis, desensitization, and recovery of sensitivity increased at the incubation temperature was increased. Arrhenius plots of the data yielded activation energies of 25, 14, and 23 kcal.mol-1 for activation, desensitization, and resensitization, respectively. These data suggest that a certain degree of membrane phospholipid fluidity is required for these processes to occur.
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UR - http://www.scopus.com/inward/citedby.url?scp=0018896622&partnerID=8YFLogxK
M3 - Article
C2 - 6246206
AN - SCOPUS:0018896622
VL - 34
SP - 1288
EP - 1295
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
SN - 0022-3042
IS - 5
ER -