The effects of temperature on muscarinic acetylcholine receptor activation, densitization, and resensitization were studied with the use of intact mouse neuroblastoma cells (clone N1E-115), which have muscarinic receptors that mediate cyclic GMP synthesis. Below 15-20°C, activation or desensitization of muscarinic receptors by carbamylcholine and recovery from desensitization (caused by carbamylcholine at 37°C) did not occur. Above these temperatures, the apparent rates of receptor-mediated cyclic GMP synthesis, desensitization, and recovery of sensitivity increased at the incubation temperature was increased. Arrhenius plots of the data yielded activation energies of 25, 14, and 23 kcal.mol-1 for activation, desensitization, and resensitization, respectively. These data suggest that a certain degree of membrane phospholipid fluidity is required for these processes to occur.
|Original language||English (US)|
|Number of pages||8|
|Journal||Journal of Neurochemistry|
|State||Published - 1980|
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience