Abstract
Solutions or sera can be subperineurally injected into rat nerve to test their neuropathologic and, especially, their demyelinating effects. In the present study we assessed the effect of the endoneurial injection technique itself. The rate of axonal degeneration and segmental demyelination increased wth extraneous movement (of the needle or the animal), increasing needle size, rapid injection, and large volumes of injectate. We showed that graded evaluation of the pathologic abnormalities of teased fibers provides a more sensitive measure of demyelination than does evaluation of transverse sections. Transverse sections provide an inadequate approach to assess the rate of axonal degeneration. Using these approaches, no difficulty was encountered in demonstrating the greatly increased demyelinating activity of 5 nmol lysolecithin (lysophosphatidylcholine) compared with lactated Ringer's solution.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 359-377 |
| Number of pages | 19 |
| Journal | Experimental Neurology |
| Volume | 77 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1982 |
Fingerprint
ASJC Scopus subject areas
- Neuroscience(all)
- Neurology
Cite this
Technique assessment of demyelination from endoneurial injection. / Dyck, Peter J; Lais, Alfred C.; Hansen, Seona M.; Sparks, Margaret F.; Low, Phillip Anson; Parthasarathy, Sampath; Baumann, Wolfgang J.
In: Experimental Neurology, Vol. 77, No. 2, 1982, p. 359-377.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Technique assessment of demyelination from endoneurial injection
AU - Dyck, Peter J
AU - Lais, Alfred C.
AU - Hansen, Seona M.
AU - Sparks, Margaret F.
AU - Low, Phillip Anson
AU - Parthasarathy, Sampath
AU - Baumann, Wolfgang J.
PY - 1982
Y1 - 1982
N2 - Solutions or sera can be subperineurally injected into rat nerve to test their neuropathologic and, especially, their demyelinating effects. In the present study we assessed the effect of the endoneurial injection technique itself. The rate of axonal degeneration and segmental demyelination increased wth extraneous movement (of the needle or the animal), increasing needle size, rapid injection, and large volumes of injectate. We showed that graded evaluation of the pathologic abnormalities of teased fibers provides a more sensitive measure of demyelination than does evaluation of transverse sections. Transverse sections provide an inadequate approach to assess the rate of axonal degeneration. Using these approaches, no difficulty was encountered in demonstrating the greatly increased demyelinating activity of 5 nmol lysolecithin (lysophosphatidylcholine) compared with lactated Ringer's solution.
AB - Solutions or sera can be subperineurally injected into rat nerve to test their neuropathologic and, especially, their demyelinating effects. In the present study we assessed the effect of the endoneurial injection technique itself. The rate of axonal degeneration and segmental demyelination increased wth extraneous movement (of the needle or the animal), increasing needle size, rapid injection, and large volumes of injectate. We showed that graded evaluation of the pathologic abnormalities of teased fibers provides a more sensitive measure of demyelination than does evaluation of transverse sections. Transverse sections provide an inadequate approach to assess the rate of axonal degeneration. Using these approaches, no difficulty was encountered in demonstrating the greatly increased demyelinating activity of 5 nmol lysolecithin (lysophosphatidylcholine) compared with lactated Ringer's solution.
UR - http://www.scopus.com/inward/record.url?scp=0019961422&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019961422&partnerID=8YFLogxK
U2 - 10.1016/0014-4886(82)90250-3
DO - 10.1016/0014-4886(82)90250-3
M3 - Article
VL - 77
SP - 359
EP - 377
JO - Experimental Neurology
JF - Experimental Neurology
SN - 0014-4886
IS - 2
ER -