Taxa of the nasal microbiome are associated with influenza-specificIgA response to live attenuated influenza vaccine

Hannah M. Salk, Whitney L. Simon, Nathanield Lambert, Richard B Kennedy, Diane E. Grill, Brian F. Kabat, Gregory A. Poland

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Live attenuated influenza vaccine (LAIV) has demonstrated varying levels of efficacy against seasonal influenza; however, LAIV may be used as a tool to measure interactions between the human microbiome and a live, replicating virus. To increase our knowledge of this interaction,we measured changes to the nasal microbiome in subjects who received LAIV to determine if associations between influenza-specific IgA production and the nasal microbiome exist after immunization with a live virus vaccine. The anterior nares of 47 healthy subjects were swabbed pre- (Day 0) and post- (Days 7 and 28) LAIV administration, and nasal washes were conducted on Days 0 and 28.We performed next-generation sequencing on amplified 16s rRNA genes and measuredmucosal influenza-specific IgA titers via enzyme-linked immunosorbent assay (ELISA). A significant increase in alpha diversity was identified (Observed,CHAO, and ACE) between Days 7 vs 0 (p-values = 0.017, 0.005, 0.005, respectively) and between Days 28 vs 0 (p-values = 0.054, 0.030, 0.050, respectively). Several significant associations between the presence of different microbial species, including Lactobacillus helveticus, Prevotella melaninogenica, Streptococcus infantis, Veillonella dispar, and Bacteroides ovatus, and influenza-specific H1 and H3 IgA antibody response were demonstrated. These data suggest that LAIV alters the nasal microbiome, allowing several less-Abundant OTUs to establish a community niche. Additionally, specific alterations in the nasal microbiome are significantly associated with variations in influenza-specific IgA antibody production and could be clinically relevant.

Original languageEnglish (US)
Article numbere0162803
JournalPLoS One
Volume11
Issue number9
DOIs
StatePublished - Sep 1 2016

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Attenuated Vaccines
Influenza Vaccines
Microbiota
Nose
influenza
Human Influenza
Immunoglobulin A
vaccines
Viruses
Antibody Formation
Prevotella melaninogenica
Lactobacillus helveticus
Veillonella
Immunization
Intranasal Administration
Immunosorbents
Bacteroides
Antibodies
Streptococcus
Veillonella dispar

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Taxa of the nasal microbiome are associated with influenza-specificIgA response to live attenuated influenza vaccine. / Salk, Hannah M.; Simon, Whitney L.; Lambert, Nathanield; Kennedy, Richard B; Grill, Diane E.; Kabat, Brian F.; Poland, Gregory A.

In: PLoS One, Vol. 11, No. 9, e0162803, 01.09.2016.

Research output: Contribution to journalArticle

Salk, Hannah M. ; Simon, Whitney L. ; Lambert, Nathanield ; Kennedy, Richard B ; Grill, Diane E. ; Kabat, Brian F. ; Poland, Gregory A. / Taxa of the nasal microbiome are associated with influenza-specificIgA response to live attenuated influenza vaccine. In: PLoS One. 2016 ; Vol. 11, No. 9.
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