TY - JOUR
T1 - Tau Protein Expression in Adult Bovine Oligodendrocytes
T2 - Functional and Pathological Significance
AU - Ksiezak-Reding, Hanna
AU - Farooq, Muhammad
AU - Yang, Liang Sheng
AU - Dickson, Dennis W.
AU - LoPresti, Patrizia
N1 - Funding Information:
The authors thank Michel Goedert for his generous gift of clone htau40 and antisera Ab189 and Ab304, Peter Davies for providing monoclonal antibodies Alz 50 and PHF-1, Lester Binder for providing Tau-1, Peter Seubert from Athena Neurosciences (San Francisco, CA, USA) for providing 12E8, and A. Van de Voorde and E. Vanmechelen from Inno-genetics (Ghent, Belgium) for providing a panel of antibodies from the AT series (AT8, AT100, AT180, and AT270). This research was supported by NIH grant NS35254, the Society for Progressive Supranuclear Palsy (grant # 411), the Alzheimer’s Association, and the National Multiple Sclerosis Society (grant PP0864 to P.L.).
PY - 2003/9
Y1 - 2003/9
N2 - In tauopathies, overexpression of tau exon 10 is linked to degeneration and abnormal tau deposition in neurons and oligodendroglia (OLGs). To compare exon 10 expression in normal neurons and OLGs, adult bovine brain was examined for the expression of tau in gray matter and cultured OLGs isolated from white matter. Using exon-specific antibodies, we found that both types of tissues abundantly expressed exon 2 but isolated OLGs had a lower expression of exons 3 and 10 when compared to gray matter. Relative expression of exons 3 and 10 did not change significantly during the in vitro maturation of OLGs for 39 days. Using a panel of well-characterized antibodies against tau, we determined that isolated OLGs contained tau phosphorylated at the Tau-1, 12E8, and PHF-1 but not the AT8, AT100, AT180, and AT270 epitopes. Tau phosphorylation status diminished during in vitro maturation, suggesting that healthy OLG processes require regulated phosphorylation of tau at specific sites. We propose that the tau isoform profile and phosphorylation status contribute to the vulnerability of OLGs in degenerative diseases linked to overexpression of exon 10.
AB - In tauopathies, overexpression of tau exon 10 is linked to degeneration and abnormal tau deposition in neurons and oligodendroglia (OLGs). To compare exon 10 expression in normal neurons and OLGs, adult bovine brain was examined for the expression of tau in gray matter and cultured OLGs isolated from white matter. Using exon-specific antibodies, we found that both types of tissues abundantly expressed exon 2 but isolated OLGs had a lower expression of exons 3 and 10 when compared to gray matter. Relative expression of exons 3 and 10 did not change significantly during the in vitro maturation of OLGs for 39 days. Using a panel of well-characterized antibodies against tau, we determined that isolated OLGs contained tau phosphorylated at the Tau-1, 12E8, and PHF-1 but not the AT8, AT100, AT180, and AT270 epitopes. Tau phosphorylation status diminished during in vitro maturation, suggesting that healthy OLG processes require regulated phosphorylation of tau at specific sites. We propose that the tau isoform profile and phosphorylation status contribute to the vulnerability of OLGs in degenerative diseases linked to overexpression of exon 10.
KW - Bovine brain
KW - Microtubule-associated protein tau
KW - Oligodendrocytes
KW - Phosphoepitopes
KW - Tau isoforms
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U2 - 10.1023/A:1024952600774
DO - 10.1023/A:1024952600774
M3 - Article
C2 - 12938862
AN - SCOPUS:0042880978
SN - 0364-3190
VL - 28
SP - 1385
EP - 1392
JO - Neurochemical Research
JF - Neurochemical Research
IS - 9
ER -