T cell epitopes of type II collagen that regulate murine collagen-induced arthritis

L. K. Myers, J. M. Seyer, J. M. Stuart, K. Terato, C. S. David, A. H. Kang

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53 Scopus citations

Abstract

Chick type II collagen (CII), a protein commonly found in joint cartilage, induces an autoimmune arthritis when administered to susceptible strains of mice. A cyanogen bromide fragment of CII, CB11, contains the requisite epitopes critical for inducing collagen-induced arthritis. If administered as a tolerogen, however, before immunization, CB11 prevents the onset of disease. Therefore, delineation of structural elements of CB11 that can regulate autoreactive T cells became the goal of this study. To delineate the structural elements of CB11 antigenic to T cells, 14 peptides containing overlapping sequences of CB11 were generated. Mononuclear cells from CII- immunized DBA/1 mice were cultured with these peptides and the resulting supernatants examined for the production of IFN-γ. Two peptides, CII 181- 209 and CII 245-270, generated the greatest responses. The ability of these two peptides to regulate arthritis was tested by administering them to neonatal DBA/1 mice as tolerogens before immunization with CII. Both peptides suppressed the incidence of arthritis whereas no other peptide used as a tolerogen significantly altered the course of the disease. T cells from four arthritis-resistant murine strains did not recognize either peptide when immunized with CII, whereas cells from the disease-susceptible B10.Q mice responded well to both. Thus, the coincidence of T cell responses to CII 181- 209 and CII 245-270 in CIA-susceptible mice and the lack of response in disease-resistant strains or CII-tolerized mice identify these two peptides as containing important T cell epitopes that regulate CIA.

Original languageEnglish (US)
Pages (from-to)500-505
Number of pages6
JournalJournal of Immunology
Volume151
Issue number1
StatePublished - Jan 1 1993

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Myers, L. K., Seyer, J. M., Stuart, J. M., Terato, K., David, C. S., & Kang, A. H. (1993). T cell epitopes of type II collagen that regulate murine collagen-induced arthritis. Journal of Immunology, 151(1), 500-505.