T cell epitopes of type II collagen that regulate murine collagen-induced arthritis

Linda K. Myers, Jerome M. Seyer, John M. Stuart, Kuniaki Terato, Chella S. David, Andrew H. Kang

Research output: Contribution to journalArticle

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Abstract

Chick type II collagen (CII), a protein commonly found in joint cartilage, induces an autoimmune arthritis when administered to susceptible strains of mice. A cyanogen bromide fragment of CII, CB11, contains the requisite epitopes critical for inducing collagen-induced arthritis. If administered as a tolerogen, however, before immunization, CB11 prevents the onset of disease. Therefore, delineation of structural elements of CB11 that can regulate autoreactive T cells became the goal of this study. To delineate the structural elements of CB11 antigenic to T cells, 14 peptides containing overlapping sequences of CB11 were generated. Mononuclear cells from CII-immunized DBA/1 mice were cultured with these peptides and the resulting supernatants examined for the production of IFN-γ. Two peptides, CII 181-209 and CII 245-270, generated the greatest responses. The ability of these two peptides to regulate arthritis was tested by administering them to neonatal DBA/1 mice as tolerogens before immunization with CII. Both peptides suppressed the incidence of arthritis whereas no other peptide used as a tolerogen significantly altered the course of the disease. T cells from four arthritis-resistant murine strains did not recognize either peptide when immunized with CII, whereas cells from the disease-susceptible B10.Q mice responded well to both. Thus, the coincidence of T cell responses to CII 181-209 and CII 245-270 in CIA-susceptible mice and the lack of response in disease-resistant strains or CII-tolerized mice identify these two peptides as containing important T cell epitopes that regulate CIA.

Original languageEnglish (US)
Pages (from-to)500-505
Number of pages6
JournalJournal of Immunology
Volume151
Issue number1
StatePublished - Jul 1 1993

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T-Lymphocyte Epitopes
Experimental Arthritis
Collagen Type II
Peptides
Arthritis
T-Lymphocytes
Inbred DBA Mouse
Immunization
Cyanogen Bromide
Cartilage
Epitopes
Joints
Incidence

ASJC Scopus subject areas

  • Immunology

Cite this

Myers, L. K., Seyer, J. M., Stuart, J. M., Terato, K., David, C. S., & Kang, A. H. (1993). T cell epitopes of type II collagen that regulate murine collagen-induced arthritis. Journal of Immunology, 151(1), 500-505.

T cell epitopes of type II collagen that regulate murine collagen-induced arthritis. / Myers, Linda K.; Seyer, Jerome M.; Stuart, John M.; Terato, Kuniaki; David, Chella S.; Kang, Andrew H.

In: Journal of Immunology, Vol. 151, No. 1, 01.07.1993, p. 500-505.

Research output: Contribution to journalArticle

Myers, LK, Seyer, JM, Stuart, JM, Terato, K, David, CS & Kang, AH 1993, 'T cell epitopes of type II collagen that regulate murine collagen-induced arthritis', Journal of Immunology, vol. 151, no. 1, pp. 500-505.
Myers LK, Seyer JM, Stuart JM, Terato K, David CS, Kang AH. T cell epitopes of type II collagen that regulate murine collagen-induced arthritis. Journal of Immunology. 1993 Jul 1;151(1):500-505.
Myers, Linda K. ; Seyer, Jerome M. ; Stuart, John M. ; Terato, Kuniaki ; David, Chella S. ; Kang, Andrew H. / T cell epitopes of type II collagen that regulate murine collagen-induced arthritis. In: Journal of Immunology. 1993 ; Vol. 151, No. 1. pp. 500-505.
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