Systemic activation of integrin αvβ3 in donors with spontaneous intracerebral hemorrhage is associated with subsequent development of vasculopathy in the heart transplant recipient

Mohamad H. Yamani, Daniel J. Cook, E. Murat Tuzcu, Philip Paul, Norman B. Ratliff, Yang Yu, Robert Hobbs, Gustavo Rincon, Corinne Bott-Silverman, Nicholas Smedira, James B. Young, Randall C. Starling

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Recipients of hearts from donors with spontaneous intracerebral hemorrhage (ICH) are at increased risk of allograft vasculopathy compared with trauma donors. We have recently shown that the vitronectin receptor (integrin αVβ3) is upregulated in transplant vasculopathy. We hypothesized that donor ICH is associated with systemic activation of αVβ3 in the donor before transplantation. Methods: We evaluated mRNA expressions of αVβ3 (TaqMan PCR) in endomyocardial biopsy samples at 1-week post-transplant in 20 recipients from ICH donors and 20 recipients from trauma donors. To investigate whether systemic activation of αVβ3 was present in the donor before transplantation, αVβ3 expression was also evaluated in the corresponding donor spleen lymphocytes. All patients underwent serial coronary intravascular ultrasound to evaluate for coronary vasculopathy. The baseline characteristics were similar except for increased donor age in the ICH Group. Results: The ICH Group showed significant increased mRNA expression of αVβ3 in the heart biopsy samples (3.8-fold, p = 0.012) and in the corresponding donor spleen lymphocytes (3.5-fold, p = 0.014) compared with the Trauma Group. At 1 year, the ICH Group also showed increased progression of coronary vasculopathy. Multivariate regression analysis found that donor lymphocytic αVβ3 mRNA expression was independently associated with increased risk of vasculopathy (odds ratio, 1.9; 95% CI, 1.21-3.98, p = 0.03). Conclusions: Our report demonstrates the presence of systemic activation of αVβ3 in donors with spontaneous intracerebral hemorrhage and its association with the subsequent development of allograft vasculopathy in the recipient.

Original languageEnglish (US)
Pages (from-to)1014-1018
Number of pages5
JournalJournal of Heart and Lung Transplantation
Volume24
Issue number8
DOIs
StatePublished - Aug 2005
Externally publishedYes

Fingerprint

Cerebral Hemorrhage
Integrins
Tissue Donors
Messenger RNA
Allografts
Transplant Recipients
Wounds and Injuries
Spleen
Transplantation
Integrin alphaVbeta3
Lymphocytes
Transplants
Biopsy
Multivariate Analysis
Odds Ratio
Regression Analysis

ASJC Scopus subject areas

  • Surgery
  • Pulmonary and Respiratory Medicine
  • Cardiology and Cardiovascular Medicine
  • Transplantation

Cite this

Systemic activation of integrin αvβ3 in donors with spontaneous intracerebral hemorrhage is associated with subsequent development of vasculopathy in the heart transplant recipient. / Yamani, Mohamad H.; Cook, Daniel J.; Tuzcu, E. Murat; Paul, Philip; Ratliff, Norman B.; Yu, Yang; Hobbs, Robert; Rincon, Gustavo; Bott-Silverman, Corinne; Smedira, Nicholas; Young, James B.; Starling, Randall C.

In: Journal of Heart and Lung Transplantation, Vol. 24, No. 8, 08.2005, p. 1014-1018.

Research output: Contribution to journalArticle

Yamani, MH, Cook, DJ, Tuzcu, EM, Paul, P, Ratliff, NB, Yu, Y, Hobbs, R, Rincon, G, Bott-Silverman, C, Smedira, N, Young, JB & Starling, RC 2005, 'Systemic activation of integrin αvβ3 in donors with spontaneous intracerebral hemorrhage is associated with subsequent development of vasculopathy in the heart transplant recipient', Journal of Heart and Lung Transplantation, vol. 24, no. 8, pp. 1014-1018. https://doi.org/10.1016/j.healun.2004.06.003
Yamani, Mohamad H. ; Cook, Daniel J. ; Tuzcu, E. Murat ; Paul, Philip ; Ratliff, Norman B. ; Yu, Yang ; Hobbs, Robert ; Rincon, Gustavo ; Bott-Silverman, Corinne ; Smedira, Nicholas ; Young, James B. ; Starling, Randall C. / Systemic activation of integrin αvβ3 in donors with spontaneous intracerebral hemorrhage is associated with subsequent development of vasculopathy in the heart transplant recipient. In: Journal of Heart and Lung Transplantation. 2005 ; Vol. 24, No. 8. pp. 1014-1018.
@article{e43e6f1c43cd44459055f4bd83357bf3,
title = "Systemic activation of integrin αvβ3 in donors with spontaneous intracerebral hemorrhage is associated with subsequent development of vasculopathy in the heart transplant recipient",
abstract = "Background: Recipients of hearts from donors with spontaneous intracerebral hemorrhage (ICH) are at increased risk of allograft vasculopathy compared with trauma donors. We have recently shown that the vitronectin receptor (integrin αVβ3) is upregulated in transplant vasculopathy. We hypothesized that donor ICH is associated with systemic activation of αVβ3 in the donor before transplantation. Methods: We evaluated mRNA expressions of αVβ3 (TaqMan PCR) in endomyocardial biopsy samples at 1-week post-transplant in 20 recipients from ICH donors and 20 recipients from trauma donors. To investigate whether systemic activation of αVβ3 was present in the donor before transplantation, αVβ3 expression was also evaluated in the corresponding donor spleen lymphocytes. All patients underwent serial coronary intravascular ultrasound to evaluate for coronary vasculopathy. The baseline characteristics were similar except for increased donor age in the ICH Group. Results: The ICH Group showed significant increased mRNA expression of αVβ3 in the heart biopsy samples (3.8-fold, p = 0.012) and in the corresponding donor spleen lymphocytes (3.5-fold, p = 0.014) compared with the Trauma Group. At 1 year, the ICH Group also showed increased progression of coronary vasculopathy. Multivariate regression analysis found that donor lymphocytic αVβ3 mRNA expression was independently associated with increased risk of vasculopathy (odds ratio, 1.9; 95{\%} CI, 1.21-3.98, p = 0.03). Conclusions: Our report demonstrates the presence of systemic activation of αVβ3 in donors with spontaneous intracerebral hemorrhage and its association with the subsequent development of allograft vasculopathy in the recipient.",
author = "Yamani, {Mohamad H.} and Cook, {Daniel J.} and Tuzcu, {E. Murat} and Philip Paul and Ratliff, {Norman B.} and Yang Yu and Robert Hobbs and Gustavo Rincon and Corinne Bott-Silverman and Nicholas Smedira and Young, {James B.} and Starling, {Randall C.}",
year = "2005",
month = "8",
doi = "10.1016/j.healun.2004.06.003",
language = "English (US)",
volume = "24",
pages = "1014--1018",
journal = "Journal of Heart and Lung Transplantation",
issn = "1053-2498",
publisher = "Elsevier USA",
number = "8",

}

TY - JOUR

T1 - Systemic activation of integrin αvβ3 in donors with spontaneous intracerebral hemorrhage is associated with subsequent development of vasculopathy in the heart transplant recipient

AU - Yamani, Mohamad H.

AU - Cook, Daniel J.

AU - Tuzcu, E. Murat

AU - Paul, Philip

AU - Ratliff, Norman B.

AU - Yu, Yang

AU - Hobbs, Robert

AU - Rincon, Gustavo

AU - Bott-Silverman, Corinne

AU - Smedira, Nicholas

AU - Young, James B.

AU - Starling, Randall C.

PY - 2005/8

Y1 - 2005/8

N2 - Background: Recipients of hearts from donors with spontaneous intracerebral hemorrhage (ICH) are at increased risk of allograft vasculopathy compared with trauma donors. We have recently shown that the vitronectin receptor (integrin αVβ3) is upregulated in transplant vasculopathy. We hypothesized that donor ICH is associated with systemic activation of αVβ3 in the donor before transplantation. Methods: We evaluated mRNA expressions of αVβ3 (TaqMan PCR) in endomyocardial biopsy samples at 1-week post-transplant in 20 recipients from ICH donors and 20 recipients from trauma donors. To investigate whether systemic activation of αVβ3 was present in the donor before transplantation, αVβ3 expression was also evaluated in the corresponding donor spleen lymphocytes. All patients underwent serial coronary intravascular ultrasound to evaluate for coronary vasculopathy. The baseline characteristics were similar except for increased donor age in the ICH Group. Results: The ICH Group showed significant increased mRNA expression of αVβ3 in the heart biopsy samples (3.8-fold, p = 0.012) and in the corresponding donor spleen lymphocytes (3.5-fold, p = 0.014) compared with the Trauma Group. At 1 year, the ICH Group also showed increased progression of coronary vasculopathy. Multivariate regression analysis found that donor lymphocytic αVβ3 mRNA expression was independently associated with increased risk of vasculopathy (odds ratio, 1.9; 95% CI, 1.21-3.98, p = 0.03). Conclusions: Our report demonstrates the presence of systemic activation of αVβ3 in donors with spontaneous intracerebral hemorrhage and its association with the subsequent development of allograft vasculopathy in the recipient.

AB - Background: Recipients of hearts from donors with spontaneous intracerebral hemorrhage (ICH) are at increased risk of allograft vasculopathy compared with trauma donors. We have recently shown that the vitronectin receptor (integrin αVβ3) is upregulated in transplant vasculopathy. We hypothesized that donor ICH is associated with systemic activation of αVβ3 in the donor before transplantation. Methods: We evaluated mRNA expressions of αVβ3 (TaqMan PCR) in endomyocardial biopsy samples at 1-week post-transplant in 20 recipients from ICH donors and 20 recipients from trauma donors. To investigate whether systemic activation of αVβ3 was present in the donor before transplantation, αVβ3 expression was also evaluated in the corresponding donor spleen lymphocytes. All patients underwent serial coronary intravascular ultrasound to evaluate for coronary vasculopathy. The baseline characteristics were similar except for increased donor age in the ICH Group. Results: The ICH Group showed significant increased mRNA expression of αVβ3 in the heart biopsy samples (3.8-fold, p = 0.012) and in the corresponding donor spleen lymphocytes (3.5-fold, p = 0.014) compared with the Trauma Group. At 1 year, the ICH Group also showed increased progression of coronary vasculopathy. Multivariate regression analysis found that donor lymphocytic αVβ3 mRNA expression was independently associated with increased risk of vasculopathy (odds ratio, 1.9; 95% CI, 1.21-3.98, p = 0.03). Conclusions: Our report demonstrates the presence of systemic activation of αVβ3 in donors with spontaneous intracerebral hemorrhage and its association with the subsequent development of allograft vasculopathy in the recipient.

UR - http://www.scopus.com/inward/record.url?scp=23744477882&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23744477882&partnerID=8YFLogxK

U2 - 10.1016/j.healun.2004.06.003

DO - 10.1016/j.healun.2004.06.003

M3 - Article

C2 - 16102435

AN - SCOPUS:23744477882

VL - 24

SP - 1014

EP - 1018

JO - Journal of Heart and Lung Transplantation

JF - Journal of Heart and Lung Transplantation

SN - 1053-2498

IS - 8

ER -