Synthesis of a technetium-99m-labeled thymidine analog: A potential HSV1-TK substrate for non-invasive reporter gene expression imaging

Yi Zhang, Xiaoman Dai, David F Kallmes, Dongfeng Pan

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

{2-[5-(2′-Fluoro-2′-deoxyuridin-5-yl)pent-4(E)-enyl] [2-(2-mercaptoethyl)aminoethyl]aminoethanethiolato(3)-N,N′,S,S′} oxo-[ 99mTc]technetium(V), a potential viral thymidine kinase substrate, was synthesized by coupling 2′-fluoro-2′-deoxyuridine analog with a N2S2 radiometal chelator, followed by [Tc-99m]technetium conjugation. The chemical structure of the radioactive probe was characterized by 1H NMR and high resolution MS using Re-188 conjugated mimics. The radiochemical purity and yield were identified as 98% and 42%, respectively.

Original languageEnglish (US)
Pages (from-to)8673-8676
Number of pages4
JournalTetrahedron Letters
Volume45
Issue number47
DOIs
StatePublished - Nov 15 2004

Fingerprint

Technetium
Reporter Genes
Gene expression
Thymidine
Gene Expression
Imaging techniques
Thymidine Kinase
Substrates
Chelating Agents
Nuclear magnetic resonance
2'fluoro-2'-deoxyuridine
Proton Magnetic Resonance Spectroscopy

Keywords

  • FTcAU
  • Nuclear imaging
  • Reporter probe
  • TK substrate

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Drug Discovery

Cite this

Synthesis of a technetium-99m-labeled thymidine analog : A potential HSV1-TK substrate for non-invasive reporter gene expression imaging. / Zhang, Yi; Dai, Xiaoman; Kallmes, David F; Pan, Dongfeng.

In: Tetrahedron Letters, Vol. 45, No. 47, 15.11.2004, p. 8673-8676.

Research output: Contribution to journalArticle

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