[3 5S]Sulfate incorporation into myelin glycoproteins I. Central nervous system

Jean Marie Matthieu, Richard H. Quarles, Joseph F. Poduslo, Roscoe O. Brady

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The in vivo incorporation of [3 5S]sulfate and [3H]fucose into rat brain myelin was investigated. Most of the 3 5S in the myelin was in sulfatide, but about 4% was associated with the residual proteins after chloroform/methanol extraction. Polyacrylamide gel electrophoresis of these proteins indicated that the major 3 5S-labeled component corresponded to the major fucose-labeled glycoprotein. The labeling of this predominant glycoprotein with sulfate was more selective than with fucose, since there was relatively little incorporation of sulfate into some of the minor fucose-labeled glycoproteins. There was little or no 3 5S associated with proteolipid or basic protein on polyacrylamide gels. The fucose-labeled glycoproteins were converted to glycopeptides by pronase digestion and separated into two major classes by gel filtration on Sephadex-G50. Only the higher molecular weight class contained significant amounts of 3 5S. The association of 3 5S with the glycopeptides was not due to binding of sulfatide or free inorganic sulfate. The results indicate that the predominant myelin-associated glycoprotein in rat brain is sulfated.

Original languageEnglish (US)
Pages (from-to)159-166
Number of pages8
JournalBBA - General Subjects
Volume392
Issue number1
DOIs
StatePublished - May 5 1975
Externally publishedYes

Fingerprint

Fucose
Neurology
Myelin Sheath
Sulfates
Glycoproteins
Central Nervous System
Sulfoglycosphingolipids
Glycopeptides
Rats
Brain
Myelin-Associated Glycoprotein
Proteolipids
Pronase
Proteins
Chloroform
Electrophoresis
Labeling
Gel Chromatography
Methanol
Polyacrylamide Gel Electrophoresis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Medicine(all)

Cite this

[3 5S]Sulfate incorporation into myelin glycoproteins I. Central nervous system. / Matthieu, Jean Marie; Quarles, Richard H.; Poduslo, Joseph F.; Brady, Roscoe O.

In: BBA - General Subjects, Vol. 392, No. 1, 05.05.1975, p. 159-166.

Research output: Contribution to journalArticle

Matthieu, Jean Marie ; Quarles, Richard H. ; Poduslo, Joseph F. ; Brady, Roscoe O. / [3 5S]Sulfate incorporation into myelin glycoproteins I. Central nervous system. In: BBA - General Subjects. 1975 ; Vol. 392, No. 1. pp. 159-166.
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