Sulochrin inhibits eosinophil activation and chemotaxis

H. Ohashi, Y. Motegi, H. Kita, G. J. Gleich, T. Miura, M. Ishikawa, H. Kawai, H. Fukamachi

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Objective: Because eosinophils likely play important roles in the pathophysiology of allergic diseases, specific inhibitors of eosinophils may be desirable to treat such diseases. To evaluate the capacity of a novel compound, sulochrin, as an inhibitor of eosinophilic inflammation, we examined the effects of this compound on various effector functions of eosinophils. Materials and methods: We examined the effects of sulochrin on degranulation of human eosinophils stimulated with platelet-activating factor (PAF) or Sepharose 4B beads coated with secretory IgA (sIgA) or IgG. The effects of sulochrin on other effector functions of human eosinophils, including superoxide anion (O2-) production, leukotriene (LT) C4 release, and interleukin (IL)-8 production induced by sIgA-beads were also studied. Finally, using PAF and LTB4 as chemoattractants, we evaluated the potency of sulochrin to inhibit eosinophil migration in vitro and in vivo. Results: Sulochrin inhibited EDN release by eosinophils stimulated with sIgA-beads, IgG-beads and PAF in a concentration-dependent manner; IC50 values were 0.75 μM, 0.30 μM and 0.03 μM. Eosinophil O2- production, LTC4 release, and IL-8 production were also inhibited by sulochrin. Furthermore, PAF-induced chemotaxis of human eosinophils and LTB4-induced chemotaxis of guinea pig eosinophils were abolished by 1 μM of sulochrin. Finally, sulochrin potently inhibited LTB4-induced infiltration of eosinophils into the skin of guinea-pig in vivo. Conclusions: These results suggest that sulochrin is a potent inhibitor of various effector functions of eosinophils. Sulochrin and its derivatives may be useful in the development of therapeutic approaches for patients with allergic diseases.

Original languageEnglish (US)
Pages (from-to)409-415
Number of pages7
JournalInflammation Research
Volume47
Issue number10
DOIs
StatePublished - 1998

Keywords

  • Chemotaxis
  • Degranulation
  • Eosinophil
  • Inhibitor
  • Sulochrin

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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    Ohashi, H., Motegi, Y., Kita, H., Gleich, G. J., Miura, T., Ishikawa, M., Kawai, H., & Fukamachi, H. (1998). Sulochrin inhibits eosinophil activation and chemotaxis. Inflammation Research, 47(10), 409-415. https://doi.org/10.1007/s000110050352