Subnuclear distribution of the vitamin d receptor

J. P. Bidwell, Andre J van Wijnen, E. G. Fey, H. Merriman, S. Penman, J. L. Stein, G. S. Stein, J. B. Lian

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The subnuclear distribution of the vitamin D receptor was investigated to begin addressing the contribution of nuclear architecture to vitamin D-responsive control of gene expression in ROS 17/2.8 rat osteosarcoma cells. The nuclear matrix is an anastomosing network of filaments that is functionally associated with DNA replication, transcription, and RNA processing. The representation of vitamin D receptor in the nuclear matrix and nonmatrix nuclear fractions was determined by the combined application of 1) sequence-specific interactions with the vitamin D receptor binding element of the rat bone-specific osteocalcin gene promoter and 2) Western blot analysis. Both methods confirmed the presence of vitamin D receptor in the nonmatrix nuclear fraction and the absence of detectable vitamin D receptors associated with the nuclear matrix. In contrast, these same nuclear matrix proteins preparations exhibited association with the general transcription factor AP-1 and a bone tissue-specific promoter binding factor NMP2. NMP-2 exhibits recognition for a promoter domain contiguous to the vitamin D-responsive element of the osteocalcin gene, although the vitamin D receptor does not appear to be a component of the nuclear matrix proteins. Interrelationships between nuclear matrix proteins and nonmatrix nuclear proteins, in mediating steroid hormone responsiveness of a vitamin D-regulated promoter, are therefore suggested.

Original languageEnglish (US)
Pages (from-to)494-500
Number of pages7
JournalJournal of Cellular Biochemistry
Volume54
Issue number4
StatePublished - 1994
Externally publishedYes

Fingerprint

Calcitriol Receptors
Vitamins
Nuclear Matrix-Associated Proteins
Nuclear Matrix
Vitamin D
Osteocalcin
Rats
Bone
Genes
Steroid hormones
General Transcription Factors
Bone and Bones
Transcription Factor AP-1
Osteosarcoma
Transcription
Nuclear Proteins
DNA Replication
Gene expression
Western Blotting
Steroids

Keywords

  • AP-1
  • NMP2
  • Nuclear matrix
  • Protein
  • Vitamin D

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Bidwell, J. P., van Wijnen, A. J., Fey, E. G., Merriman, H., Penman, S., Stein, J. L., ... Lian, J. B. (1994). Subnuclear distribution of the vitamin d receptor. Journal of Cellular Biochemistry, 54(4), 494-500.

Subnuclear distribution of the vitamin d receptor. / Bidwell, J. P.; van Wijnen, Andre J; Fey, E. G.; Merriman, H.; Penman, S.; Stein, J. L.; Stein, G. S.; Lian, J. B.

In: Journal of Cellular Biochemistry, Vol. 54, No. 4, 1994, p. 494-500.

Research output: Contribution to journalArticle

Bidwell, JP, van Wijnen, AJ, Fey, EG, Merriman, H, Penman, S, Stein, JL, Stein, GS & Lian, JB 1994, 'Subnuclear distribution of the vitamin d receptor', Journal of Cellular Biochemistry, vol. 54, no. 4, pp. 494-500.
Bidwell JP, van Wijnen AJ, Fey EG, Merriman H, Penman S, Stein JL et al. Subnuclear distribution of the vitamin d receptor. Journal of Cellular Biochemistry. 1994;54(4):494-500.
Bidwell, J. P. ; van Wijnen, Andre J ; Fey, E. G. ; Merriman, H. ; Penman, S. ; Stein, J. L. ; Stein, G. S. ; Lian, J. B. / Subnuclear distribution of the vitamin d receptor. In: Journal of Cellular Biochemistry. 1994 ; Vol. 54, No. 4. pp. 494-500.
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