Subnuclear distribution of the vitamin D receptor

Joseph P. Bidwell, André J. van Wijnen, Edward G. Fey, Harold Merriman, Sheldon Penman, Janet L. Stein, Gary S. Stein, Jane B. Lian

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10 Scopus citations

Abstract

The subnuclear distribution of the vitamin D receptor was investigated to begin addressing the contribution of nuclear architecture to vitamin D–responsive control of gene expression in ROS 17/2.8 rat osteosarcoma cells. The nuclear matrix is an anastomosing network of filaments that is functionally associated with DNA replication, transcription, and RNA processing. The representation of vitamin D receptor in the nuclear matrix and nonmatrix nuclear fractions was determined by the combined application of (1) sequence‐specific interactions with the vitamin D receptor binding element of the rat bone‐specific osteocalcin gene promoter and (2) Western blot analysis. Both methods confirmed the presence of vitamin D receptor in the nonmatrix nuclear fraction and the absence of detectable vitamin D receptors associated with the nuclear matrix. In contrast, these same nuclear matrix proteins preparations exhibited association with the general transcription factor AP‐1 and a bone tissue‐specific promoter binding factor NMP2. NMP‐2 exhibits recognition for a promoter domain contiguous to the vitamin D‐responsive element of the osteocalcin gene, although the vitamin D receptor does not appear to be a component of the nuclear matrix proteins. Interrelationships between nuclear matrix proteins and nonmatrix nuclear proteins, in mediating steroid hormone responsiveness of a vitamin D–regulated promoter, are therefore suggested.

Original languageEnglish (US)
Pages (from-to)494-500
Number of pages7
JournalJournal of cellular biochemistry
Volume54
Issue number4
DOIs
StatePublished - Apr 1994

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Keywords

  • AP‐1
  • NMP2
  • nuclear matrix
  • protein
  • vitamin D

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Bidwell, J. P., van Wijnen, A. J., Fey, E. G., Merriman, H., Penman, S., Stein, J. L., Stein, G. S., & Lian, J. B. (1994). Subnuclear distribution of the vitamin D receptor. Journal of cellular biochemistry, 54(4), 494-500. https://doi.org/10.1002/jcb.240540417