Studies to improve fixation of human nerves. Part 3. Effect of osmolality of glutaraldehyde solutions on relationship of axonal area to number of myelin lamellae

A. Ohnishi, P. C. O'Brien, P. J. Dyck

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

When rat peroneal nerves were fixed under 5 different fixation schedules with various osmolalities (310, 410 and 900 milliosmoles), the frequency of abnormality of mitochondria in the axoplasm was lowest in myelinated fibers fixed with 6% glutaraldehyde in 0.1 M cacodylate buffer (900 milliosmoles). The relationship of axonal area to number of myelin lamellae was significantly different between high-osmolality fixation and low-osmolality fixation. Assuming that the number of myelin lamellae remains the same with fixation, there was a significant shrinkage in the axonal area of fibers with high-osmolality fixation. The amount of shrinkage was less in larger fibers than in smaller fibers fixed at high osmolality.

Original languageEnglish (US)
Pages (from-to)193-199
Number of pages7
JournalJournal of the neurological sciences
Volume27
Issue number2
DOIs
StatePublished - Feb 1976

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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