TY - JOUR
T1 - Studies to improve fixation of human nerves. Part 3. Effect of osmolality of glutaraldehyde solutions on relationship of axonal area to number of myelin lamellae
AU - Ohnishi, A.
AU - O'Brien, P. C.
AU - Dyck, P. J.
PY - 1976/2
Y1 - 1976/2
N2 - When rat peroneal nerves were fixed under 5 different fixation schedules with various osmolalities (310, 410 and 900 milliosmoles), the frequency of abnormality of mitochondria in the axoplasm was lowest in myelinated fibers fixed with 6% glutaraldehyde in 0.1 M cacodylate buffer (900 milliosmoles). The relationship of axonal area to number of myelin lamellae was significantly different between high-osmolality fixation and low-osmolality fixation. Assuming that the number of myelin lamellae remains the same with fixation, there was a significant shrinkage in the axonal area of fibers with high-osmolality fixation. The amount of shrinkage was less in larger fibers than in smaller fibers fixed at high osmolality.
AB - When rat peroneal nerves were fixed under 5 different fixation schedules with various osmolalities (310, 410 and 900 milliosmoles), the frequency of abnormality of mitochondria in the axoplasm was lowest in myelinated fibers fixed with 6% glutaraldehyde in 0.1 M cacodylate buffer (900 milliosmoles). The relationship of axonal area to number of myelin lamellae was significantly different between high-osmolality fixation and low-osmolality fixation. Assuming that the number of myelin lamellae remains the same with fixation, there was a significant shrinkage in the axonal area of fibers with high-osmolality fixation. The amount of shrinkage was less in larger fibers than in smaller fibers fixed at high osmolality.
UR - http://www.scopus.com/inward/record.url?scp=0017239412&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017239412&partnerID=8YFLogxK
U2 - 10.1016/0022-510X(76)90061-7
DO - 10.1016/0022-510X(76)90061-7
M3 - Article
C2 - 814207
AN - SCOPUS:0017239412
VL - 27
SP - 193
EP - 199
JO - Journal of the Neurological Sciences
JF - Journal of the Neurological Sciences
SN - 0022-510X
IS - 2
ER -