TY - JOUR
T1 - Studies to improve fixation of human nerves. Part 3. Effect of osmolality of glutaraldehyde solutions on relationship of axonal area to number of myelin lamellae
AU - Ohnishi, A.
AU - O'Brien, P. C.
AU - Dyck, P. J.
N1 - Funding Information:
The studies by Sabatini, Bensch and Barnett (1963) indicated that the concentration of glutaraldehyde in fixative solutions could be changed over a wide range without marked effect on ultrastructure. Subsequently, it was discovered that the concentration of glutaraldehyde, the inclusion of other constituents, and the osmolality of the fixative solution might affect ultrastructure (Maunsbach 1966; Hayat 1970). In assessing the effect of osmolality from constituents of fixing solutions, it is difficult to isolate the effect of each of them because they are interdependent. In the present study, the effects of various glutaraldehyde fixative solutions, of high and low os- This investigation was supported in part by MDA-8, Research Grants NS-5811, NS-7541, and AM2-2200 from the National Institutes of Health, Public Health Service and by grants from the Upton and Heller Funds.
PY - 1976/2
Y1 - 1976/2
N2 - When rat peroneal nerves were fixed under 5 different fixation schedules with various osmolalities (310, 410 and 900 milliosmoles), the frequency of abnormality of mitochondria in the axoplasm was lowest in myelinated fibers fixed with 6% glutaraldehyde in 0.1 M cacodylate buffer (900 milliosmoles). The relationship of axonal area to number of myelin lamellae was significantly different between high-osmolality fixation and low-osmolality fixation. Assuming that the number of myelin lamellae remains the same with fixation, there was a significant shrinkage in the axonal area of fibers with high-osmolality fixation. The amount of shrinkage was less in larger fibers than in smaller fibers fixed at high osmolality.
AB - When rat peroneal nerves were fixed under 5 different fixation schedules with various osmolalities (310, 410 and 900 milliosmoles), the frequency of abnormality of mitochondria in the axoplasm was lowest in myelinated fibers fixed with 6% glutaraldehyde in 0.1 M cacodylate buffer (900 milliosmoles). The relationship of axonal area to number of myelin lamellae was significantly different between high-osmolality fixation and low-osmolality fixation. Assuming that the number of myelin lamellae remains the same with fixation, there was a significant shrinkage in the axonal area of fibers with high-osmolality fixation. The amount of shrinkage was less in larger fibers than in smaller fibers fixed at high osmolality.
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U2 - 10.1016/0022-510X(76)90061-7
DO - 10.1016/0022-510X(76)90061-7
M3 - Article
C2 - 814207
AN - SCOPUS:0017239412
VL - 27
SP - 193
EP - 199
JO - Journal of the Neurological Sciences
JF - Journal of the Neurological Sciences
SN - 0022-510X
IS - 2
ER -