Structure and functional connections of presynaptic terminals in the vertebrate retina revealed by activity-dependent dyes and confocal microscopy

Robert F. Miller, Mary H. Fagerson, Nathan P. Staff, Robert Wolfe, Terry Doerr, Jon Gottesman, Michael A. Sikora, Rachel Schuneman

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The fluorescent dyes sulforhodamine 101 (SR 101) and FM1-43 were used as activity-dependent dyes (ADDs) to label presynaptic terminals in the retinas of a broad range of animals, including amphibians, mammals, fish, and turtles. The pattern of dye uptake was studied in live retinal preparations by using brightfield, fluorescence, and confocal microscopy. When bath-applied to the retina-eyecup, these dyes were avidly sequestered by the presynaptic terminals of virtually all rods, cones, and bipolar and amacrine cells; ganglion cell dendrites and horizontal cells lacked significant dye accumulation. Other structures stained with these dyes included pigment epithelial cells, cone outer segments, and Müller cell end-feet. Studies of dye uptake in dark- and light-adapted preparations showed significant differences in the dye accumulation pattern in the inner plexiform layer (IPL), suggesting a dynamic, light-modulated control of endocytotic activity. Presynaptic terminals in the IPL could be segregated on the basis of volume: bipolar varicosities in the IPL were typically larger than those of amacrine cells. The combination of retrograde labeling of ganglion cells and presynaptic terminal labeling with ADDs served as the experimental preparation for three-dimensional reconstruction of both structures, based on dual detector, confocal microscopy. Our results demonstrate a new approach for studying synaptic interactions in retinal function. These findings provide new insights into the likely number and position of functional connections from amacrine and bipolar cell terminals onto ganglion cell dendrites.

Original languageEnglish (US)
Pages (from-to)129-155
Number of pages27
JournalJournal of Comparative Neurology
Volume437
Issue number2
DOIs
StatePublished - Aug 20 2001

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Keywords

  • Amacrine cells
  • Bipolar cells
  • FM1-43
  • Photoreceptors
  • Retina
  • Sulforhodamine 101

ASJC Scopus subject areas

  • Neuroscience(all)

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