Structure-activity relationships in rodent diaphragm muscle fibers vs. neuromuscular junctions

Dylan C. Sieck; Wen Zhi Zhan; Yun Hua Fang; Leonid G. Ermilov; Gary C. Sieck; Carlos B. Mantilla

doi:10.1016/j.resp.2011.10.015

Structure-activity relationships in rodent diaphragm muscle fibers vs. neuromuscular junctions

Dylan C. Sieck, Wen Zhi Zhan, Yun Hua Fang, Leonid G. Ermilov, Gary C. Sieck, Carlos B. Mantilla

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

The diaphragm muscle (DIAm) is a highly active muscle of mixed fiber type composition. We hypothesized that consistent with greater activation history and proportion of fatigue-resistant fibers, neuromuscular transmission failure is lower in the mouse compared to the rat DIAm, and that neuromuscular junction (NMJ) morphology will match their different functional demands. Minute ventilation and duty cycle were higher in the mouse than in the rat. The proportion of fatigue-resistant fibers was similar in the rat and mouse; however the contribution of fatigue-resistant fibers to total DIAm mass was higher in the mouse. Neuromuscular transmission failure was less in mice than in rats. Motor end-plate area differed across fibers in rat but not in mouse DIAm, where NMJs displayed greater complexity overall. Thus, differences across species in activation history and susceptibility to neuromuscular transmission failure are reflected in the relative contribution of fatigue resistant muscle fibers to total DIAm mass, but not in type-dependent morphological differences at the NMJ.

Original language	English (US)
Pages (from-to)	88-96
Number of pages	9
Journal	Respiratory Physiology and Neurobiology
Volume	180
Issue number	1
DOIs	https://doi.org/10.1016/j.resp.2011.10.015
State	Published - Jan 15 2012

Keywords

Fiber type
Morphometry
Motor unit
Plethysmography
Respiratory muscles

ASJC Scopus subject areas

Neuroscience(all)
Physiology
Pulmonary and Respiratory Medicine

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10.1016/j.resp.2011.10.015

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title = "Structure-activity relationships in rodent diaphragm muscle fibers vs. neuromuscular junctions",

abstract = "The diaphragm muscle (DIAm) is a highly active muscle of mixed fiber type composition. We hypothesized that consistent with greater activation history and proportion of fatigue-resistant fibers, neuromuscular transmission failure is lower in the mouse compared to the rat DIAm, and that neuromuscular junction (NMJ) morphology will match their different functional demands. Minute ventilation and duty cycle were higher in the mouse than in the rat. The proportion of fatigue-resistant fibers was similar in the rat and mouse; however the contribution of fatigue-resistant fibers to total DIAm mass was higher in the mouse. Neuromuscular transmission failure was less in mice than in rats. Motor end-plate area differed across fibers in rat but not in mouse DIAm, where NMJs displayed greater complexity overall. Thus, differences across species in activation history and susceptibility to neuromuscular transmission failure are reflected in the relative contribution of fatigue resistant muscle fibers to total DIAm mass, but not in type-dependent morphological differences at the NMJ.",

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note = "Funding Information: Supported by NIH grants HL096750 , AR051173 , and a Career Development Award from Mayo Clinic.",

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AU - Sieck, Gary C.

AU - Mantilla, Carlos B.

N1 - Funding Information: Supported by NIH grants HL096750 , AR051173 , and a Career Development Award from Mayo Clinic.

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AB - The diaphragm muscle (DIAm) is a highly active muscle of mixed fiber type composition. We hypothesized that consistent with greater activation history and proportion of fatigue-resistant fibers, neuromuscular transmission failure is lower in the mouse compared to the rat DIAm, and that neuromuscular junction (NMJ) morphology will match their different functional demands. Minute ventilation and duty cycle were higher in the mouse than in the rat. The proportion of fatigue-resistant fibers was similar in the rat and mouse; however the contribution of fatigue-resistant fibers to total DIAm mass was higher in the mouse. Neuromuscular transmission failure was less in mice than in rats. Motor end-plate area differed across fibers in rat but not in mouse DIAm, where NMJs displayed greater complexity overall. Thus, differences across species in activation history and susceptibility to neuromuscular transmission failure are reflected in the relative contribution of fatigue resistant muscle fibers to total DIAm mass, but not in type-dependent morphological differences at the NMJ.

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Structure-activity relationships in rodent diaphragm muscle fibers vs. neuromuscular junctions

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Keywords

ASJC Scopus subject areas

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