Structural alterations of acidic proteins by acid treatment of chromatin

T. C. Spelsberg, W. M. Mitchell, F. Chytil

Research output: Contribution to journalLetter

7 Scopus citations

Abstract

Recent studies into the properties and biological function of the acidic (non-histone) chromatin proteins have utilized inorganic or organic acids to first remove the histones prior to analysis of the acidic proteins. Examination of the effects of the acid treatment on the DNA and acidic proteins by immunochemistry, circular dichroism, and the ability of the DNA to serve as a template in the in vitro DNA-dependent RNA synthesis, has demonstrated a marked structural change (denaturation) in the proteins and DNA after the acid treatment. Other methods of removing histones, e.g., by high salt or salt and urea, are recommended for studies, especially for those of the biological functions, of the DNA and acidic proteins.

Original languageEnglish (US)
Pages (from-to)243-246
Number of pages4
JournalMolecular and Cellular Biochemistry
Volume1
Issue number2
DOIs
StatePublished - Sep 1 1973

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

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