Strong evidence of a genetic determinant for mammographic density, a major risk factor for breast cancer

Celine M. Vachon, Thomas A. Sellers, Erin E. Carlson, Julie M. Cunningham, Christopher A. Hilker, Regenia L. Smalley, Daniel J. Schaid, Linda E. Kelemen, Fergus J. Couch, V. Shane Pankratz

Research output: Contribution to journalArticlepeer-review

61 Scopus citations

Abstract

Increased mammographic density (MD), the proportion of dense tissue visible on a mammogram, is a strong risk factor for breast cancer, common in the population and clusters in families. We conducted the first genome-wide linkage scan to identify genes influencing MD. DNA was obtained from 889 relatives (756 women, 133 men) from 89 families. Percent MD was estimated on 618 (82%) female family members using a validated computer-assisted thresholding method. The genome-wide scan included 403 microsatellite DNA markers with an average spacing of 9 cM. Fine mapping of a region of chromosome 5p (5p13.1-5p15.1) was done using 21 additional closely spaced DNA markers. Linkage analyses were conducted to quantify the evidence for a gene responsible for MD across the genome. The maximum log odds for linkage (LOD) score from the genome-wide scan was on chromosome 5p (LOD = 2.9, supporting linkage by a factor of 102.9 or 794 to 1) with a 1-LOD interval spanning 28.6 cM. Two suggestive regions for linkage were also identified on chromosome 12 (LOD = 2.6, 1-LOD interval of 14.8 cM; and LOD = 2.5, 1-LOD interval of 17.2 cM). Finer mapping of the region surrounding the maximum LOD on chromosome 5p resulted in stronger and statistically significant evidence for linkage (LOD = 4.2) and a narrowed 1-LOD interval (13.4 cM). The putative locus on chromosome 5p is likely to account for up to 22% of variation in MD. Hence, 1 or more of the 45 candidate genes in this region could explain a large proportion of MD and, potentially, breast cancer.

Original languageEnglish (US)
Pages (from-to)8412-8418
Number of pages7
JournalCancer research
Volume67
Issue number17
DOIs
StatePublished - Sep 1 2007

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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