Stem cells and the developing mammary gland

Maisam Makarem, Benjamin T. Spike, Christopher Dravis, Nagarajan Kannan, Geoffrey M. Wahl, Connie J. Eaves

Research output: Contribution to journalReview article

25 Citations (Scopus)

Abstract

The mammary gland undergoes dynamic changes throughout life. In the mouse, these begin with initial morphogenesis of the gland in the mid-gestation embryo followed by hormonally regulated changes during puberty and later in adulthood. The adult mammary gland contains a hierarchy of cell types with varying potentials for self-maintenance and differentiation. These include cells able to produce complete, functional mammary glands in vivo and that contain daughter cells with the same remarkable regenerative potential, as well as cells with more limited clonogenic activity in vitro. Here we review how applying in vitro and in vivo methods for quantifying these cells in adult mammary tissue to fetal mammary cells has enabled the first cells fulfilling the functional criteria of transplantable, isolated mammary stem cells to be identified a few days before birth. Thereafter, the number of these cells increases rapidly. Populations containing these fetal stem cells display growth and gene expression programs that differ from their adult counterparts but share signatures characteristic of certain types of breast cancer. Such observations reinforce growing evidence of important differences between tissue-specific fetal and adult cells with stem cell properties and emphasize the merits of investigating their molecular basis.

Original languageEnglish (US)
Pages (from-to)209-219
Number of pages11
JournalJournal of Mammary Gland Biology and Neoplasia
Volume18
Issue number2
DOIs
StatePublished - Jun 1 2013
Externally publishedYes

Fingerprint

Human Mammary Glands
Stem Cells
Breast
Fetus
Fetal Stem Cells
Puberty
Morphogenesis
Embryonic Structures
Cell Count
Parturition
Breast Neoplasms
Gene Expression
Pregnancy
Growth
Population

Keywords

  • Breast cancer
  • Development
  • Fetal
  • Mammary gland
  • Progenitors
  • Stem cells
  • Transcriptomes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Makarem, M., Spike, B. T., Dravis, C., Kannan, N., Wahl, G. M., & Eaves, C. J. (2013). Stem cells and the developing mammary gland. Journal of Mammary Gland Biology and Neoplasia, 18(2), 209-219. https://doi.org/10.1007/s10911-013-9284-6

Stem cells and the developing mammary gland. / Makarem, Maisam; Spike, Benjamin T.; Dravis, Christopher; Kannan, Nagarajan; Wahl, Geoffrey M.; Eaves, Connie J.

In: Journal of Mammary Gland Biology and Neoplasia, Vol. 18, No. 2, 01.06.2013, p. 209-219.

Research output: Contribution to journalReview article

Makarem, M, Spike, BT, Dravis, C, Kannan, N, Wahl, GM & Eaves, CJ 2013, 'Stem cells and the developing mammary gland', Journal of Mammary Gland Biology and Neoplasia, vol. 18, no. 2, pp. 209-219. https://doi.org/10.1007/s10911-013-9284-6
Makarem, Maisam ; Spike, Benjamin T. ; Dravis, Christopher ; Kannan, Nagarajan ; Wahl, Geoffrey M. ; Eaves, Connie J. / Stem cells and the developing mammary gland. In: Journal of Mammary Gland Biology and Neoplasia. 2013 ; Vol. 18, No. 2. pp. 209-219.
@article{056775259e614937b6f887ee9ea435d9,
title = "Stem cells and the developing mammary gland",
abstract = "The mammary gland undergoes dynamic changes throughout life. In the mouse, these begin with initial morphogenesis of the gland in the mid-gestation embryo followed by hormonally regulated changes during puberty and later in adulthood. The adult mammary gland contains a hierarchy of cell types with varying potentials for self-maintenance and differentiation. These include cells able to produce complete, functional mammary glands in vivo and that contain daughter cells with the same remarkable regenerative potential, as well as cells with more limited clonogenic activity in vitro. Here we review how applying in vitro and in vivo methods for quantifying these cells in adult mammary tissue to fetal mammary cells has enabled the first cells fulfilling the functional criteria of transplantable, isolated mammary stem cells to be identified a few days before birth. Thereafter, the number of these cells increases rapidly. Populations containing these fetal stem cells display growth and gene expression programs that differ from their adult counterparts but share signatures characteristic of certain types of breast cancer. Such observations reinforce growing evidence of important differences between tissue-specific fetal and adult cells with stem cell properties and emphasize the merits of investigating their molecular basis.",
keywords = "Breast cancer, Development, Fetal, Mammary gland, Progenitors, Stem cells, Transcriptomes",
author = "Maisam Makarem and Spike, {Benjamin T.} and Christopher Dravis and Nagarajan Kannan and Wahl, {Geoffrey M.} and Eaves, {Connie J.}",
year = "2013",
month = "6",
day = "1",
doi = "10.1007/s10911-013-9284-6",
language = "English (US)",
volume = "18",
pages = "209--219",
journal = "Journal of Mammary Gland Biology and Neoplasia",
issn = "1083-3021",
publisher = "Springer New York",
number = "2",

}

TY - JOUR

T1 - Stem cells and the developing mammary gland

AU - Makarem, Maisam

AU - Spike, Benjamin T.

AU - Dravis, Christopher

AU - Kannan, Nagarajan

AU - Wahl, Geoffrey M.

AU - Eaves, Connie J.

PY - 2013/6/1

Y1 - 2013/6/1

N2 - The mammary gland undergoes dynamic changes throughout life. In the mouse, these begin with initial morphogenesis of the gland in the mid-gestation embryo followed by hormonally regulated changes during puberty and later in adulthood. The adult mammary gland contains a hierarchy of cell types with varying potentials for self-maintenance and differentiation. These include cells able to produce complete, functional mammary glands in vivo and that contain daughter cells with the same remarkable regenerative potential, as well as cells with more limited clonogenic activity in vitro. Here we review how applying in vitro and in vivo methods for quantifying these cells in adult mammary tissue to fetal mammary cells has enabled the first cells fulfilling the functional criteria of transplantable, isolated mammary stem cells to be identified a few days before birth. Thereafter, the number of these cells increases rapidly. Populations containing these fetal stem cells display growth and gene expression programs that differ from their adult counterparts but share signatures characteristic of certain types of breast cancer. Such observations reinforce growing evidence of important differences between tissue-specific fetal and adult cells with stem cell properties and emphasize the merits of investigating their molecular basis.

AB - The mammary gland undergoes dynamic changes throughout life. In the mouse, these begin with initial morphogenesis of the gland in the mid-gestation embryo followed by hormonally regulated changes during puberty and later in adulthood. The adult mammary gland contains a hierarchy of cell types with varying potentials for self-maintenance and differentiation. These include cells able to produce complete, functional mammary glands in vivo and that contain daughter cells with the same remarkable regenerative potential, as well as cells with more limited clonogenic activity in vitro. Here we review how applying in vitro and in vivo methods for quantifying these cells in adult mammary tissue to fetal mammary cells has enabled the first cells fulfilling the functional criteria of transplantable, isolated mammary stem cells to be identified a few days before birth. Thereafter, the number of these cells increases rapidly. Populations containing these fetal stem cells display growth and gene expression programs that differ from their adult counterparts but share signatures characteristic of certain types of breast cancer. Such observations reinforce growing evidence of important differences between tissue-specific fetal and adult cells with stem cell properties and emphasize the merits of investigating their molecular basis.

KW - Breast cancer

KW - Development

KW - Fetal

KW - Mammary gland

KW - Progenitors

KW - Stem cells

KW - Transcriptomes

UR - http://www.scopus.com/inward/record.url?scp=84879685072&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84879685072&partnerID=8YFLogxK

U2 - 10.1007/s10911-013-9284-6

DO - 10.1007/s10911-013-9284-6

M3 - Review article

VL - 18

SP - 209

EP - 219

JO - Journal of Mammary Gland Biology and Neoplasia

JF - Journal of Mammary Gland Biology and Neoplasia

SN - 1083-3021

IS - 2

ER -