Steady-state effects of vitronectin and fibronectin on the binding, uptake, and degradation of Pneumocystis carinii by rat alveolar macrophages

Jacques S. Hoyte, Joseph E. Standing, Andrew Harold Limper

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Pneumocystis carinii pneumonia remains a serious complication of immunodeficiency. Vitronectin (VN) and fibronectin (FN) accumulate in the lung during P. carinii infection and bind to the organism, thereby enhancing macrophage release of TNFα. It is not known whether VN and FN also regulate uptake and degradation of P. carinii by macrophages when present in concentrations similar to those in the lung during pneumonia. To address this, macrophages were cultured with 35S-radiolabeled P. carinii and organism binding, phagocytosis, and degradation determined in media alone (control), or in the presence of VN or FN (100 μg/ml each). Soluble VN and FN, in concentrations similar to those in the host, did not significantly affect binding, uptake or degradation of P. carinii by alveolar macrophages. Thus, although VN and FN enhance macrophage activation during P. carinii pneumonia, phagocytosis of the organism is not increased by these host glycoproteins under steady-state conditions.

Original languageEnglish (US)
Pages (from-to)335-345
Number of pages11
JournalInflammation
Volume21
Issue number3
DOIs
StatePublished - 1997

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Vitronectin
Pneumocystis carinii
Alveolar Macrophages
Fibronectins
Pneumocystis Pneumonia
Macrophages
Phagocytosis
Pneumocystis Infections
Lung
Macrophage Activation
Pneumonia
Glycoproteins

ASJC Scopus subject areas

  • Cell Biology
  • Immunology
  • Medicine(all)

Cite this

Steady-state effects of vitronectin and fibronectin on the binding, uptake, and degradation of Pneumocystis carinii by rat alveolar macrophages. / Hoyte, Jacques S.; Standing, Joseph E.; Limper, Andrew Harold.

In: Inflammation, Vol. 21, No. 3, 1997, p. 335-345.

Research output: Contribution to journalArticle

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