TY - JOUR
T1 - Statistical approach to estimate vaccinia-specific neutralizing antibody titers using a high-throughput assay
AU - Kennedy, Richard
AU - Pankratz, V. Shane
AU - Swanson, Eric
AU - Watson, David
AU - Golding, Hana
AU - Poland, Gregory A.
PY - 2009/8
Y1 - 2009/8
N2 - Because of the bioterrorism threat posed by agents such as variola virus, considerable time, resources, and effort have been devoted to biodefense preparation. One avenue of this research has been the development of rapid, sensitive, high-throughput assays to validate immune responses to poxviruses. Here we describe the adaptation of a β-galactosidase reporter-based vaccinia virus neutralization assay to large-scale use in a study that included over 1,000 subjects. We also describe the statistical methods involved in analyzing the large quantity of data generated. The assay and its associated methods should prove useful tools in monitoring immune responses to next-generation smallpox vaccines, studying poxvirus immunity, and evaluating therapeutic agents such as vaccinia virus immune globulin.
AB - Because of the bioterrorism threat posed by agents such as variola virus, considerable time, resources, and effort have been devoted to biodefense preparation. One avenue of this research has been the development of rapid, sensitive, high-throughput assays to validate immune responses to poxviruses. Here we describe the adaptation of a β-galactosidase reporter-based vaccinia virus neutralization assay to large-scale use in a study that included over 1,000 subjects. We also describe the statistical methods involved in analyzing the large quantity of data generated. The assay and its associated methods should prove useful tools in monitoring immune responses to next-generation smallpox vaccines, studying poxvirus immunity, and evaluating therapeutic agents such as vaccinia virus immune globulin.
UR - http://www.scopus.com/inward/record.url?scp=68149161001&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=68149161001&partnerID=8YFLogxK
U2 - 10.1128/CVI.00109-09
DO - 10.1128/CVI.00109-09
M3 - Article
C2 - 19535540
AN - SCOPUS:68149161001
SN - 1556-6811
VL - 16
SP - 1105
EP - 1112
JO - Clinical and Vaccine Immunology
JF - Clinical and Vaccine Immunology
IS - 8
ER -