Statistical approach to estimate vaccinia-specific neutralizing antibody titers using a high-throughput assay

Richard Kennedy, V. Shane Pankratz, Eric Swanson, David Watson, Hana Golding, Gregory A. Poland

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Because of the bioterrorism threat posed by agents such as variola virus, considerable time, resources, and effort have been devoted to biodefense preparation. One avenue of this research has been the development of rapid, sensitive, high-throughput assays to validate immune responses to poxviruses. Here we describe the adaptation of a β-galactosidase reporter-based vaccinia virus neutralization assay to large-scale use in a study that included over 1,000 subjects. We also describe the statistical methods involved in analyzing the large quantity of data generated. The assay and its associated methods should prove useful tools in monitoring immune responses to next-generation smallpox vaccines, studying poxvirus immunity, and evaluating therapeutic agents such as vaccinia virus immune globulin.

Original languageEnglish (US)
Pages (from-to)1105-1112
Number of pages8
JournalClinical and Vaccine Immunology
Volume16
Issue number8
DOIs
StatePublished - Aug 2009

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

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