Stable isotope labeling with amino acids in cell culture (SILAC) for studying dynamics of protein abundance and posttranslational modifications.

Ramars Amanchy, Dario Eluan Kalume, Akhilesh Pandey

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

Stable isotope labeling with amino acids in cell culture (SILAC) is a simple and straightforward approach for in vivo incorporation of a tag into proteins for relative quantitation by mass spectrometry. SILAC is a simple, yet powerful, method for investigating the dynamics of protein abundance and posttranslational modifications. Here, we provide detailed instructions for using this method to study protein complexes, protein-protein interactions, and the dynamics of protein abundance and posttranslational modifications. We expect that SILAC will become a routine technique because of its applicability to most areas of cell biology. We have also developed a Web site (http://www.silac.org) to provide researchers with updated information about this method and related resources.

Original languageEnglish (US)
Pages (from-to)pl2
JournalScience's STKE : signal transduction knowledge environment
Volume2005
Issue number267
DOIs
StatePublished - Jan 18 2005

ASJC Scopus subject areas

  • Medicine(all)

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