Stability of serum prostate-specific antigen determination across laboratory, assay, and storage time

Steven J. Jacobsen, George G. Klee, Hans Lilja, George L. Wright, Joseph E. Oesterling

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Objectives: To understand the comparability of serum prostate-specific antigen (PSA) determinations across assays and storage time. Methods: Serum PSA levels were determined for men aged 40 to 79 years from the clinical subset of the Olmsted County Study of Urinary Symptoms and Health Status Among Men on fresh samples and after a median of 32 months on banked samples, frozen at -70 °C. Baseline serum PSA levels were determined by Tandem-R PSA assay. Follow-up levels on the banked samples were determined by the IMx PSA assay and a repeat Tandem-R PSA assay in a different laboratory and by an immunofluorometric PSA assay at another site. Results: The median serum PSA level determined by Tandem-R assay at baseline was 1.0 ng/mL (25th percentile, 0.6; 75th percentile, 1.7). The distributions of determination made by follow-up Tandem-R, IMx, and immunofluorometric analyses were essentially identical. Overall, the assays were highly correlated. The correlations between the baseline serum PSA determination and repeated Tandem-R, IMx, and immunofluorometric determinations were 0.96, 0.96, and 0.97, respectively (all P < 0.001). The median duration of frozen storage was 32 months (range, 26 to 39 months), and the correlations between baseline and follow-up determinations did not change when stratified by duration of storage. Conclusions: These data provide important reassurance about the use of serum PSA determinations obtained by different assays, in different laboratories, and in properly tored samples across time.

Original languageEnglish (US)
Pages (from-to)447-453
Number of pages7
JournalUrology
Volume45
Issue number3
DOIs
StatePublished - 1995

Fingerprint

Prostate-Specific Antigen
Serum
Tandem Repeat Sequences
Health Status

ASJC Scopus subject areas

  • Urology

Cite this

Jacobsen, S. J., Klee, G. G., Lilja, H., Wright, G. L., & Oesterling, J. E. (1995). Stability of serum prostate-specific antigen determination across laboratory, assay, and storage time. Urology, 45(3), 447-453. https://doi.org/10.1016/S0090-4295(99)80014-4

Stability of serum prostate-specific antigen determination across laboratory, assay, and storage time. / Jacobsen, Steven J.; Klee, George G.; Lilja, Hans; Wright, George L.; Oesterling, Joseph E.

In: Urology, Vol. 45, No. 3, 1995, p. 447-453.

Research output: Contribution to journalArticle

Jacobsen, SJ, Klee, GG, Lilja, H, Wright, GL & Oesterling, JE 1995, 'Stability of serum prostate-specific antigen determination across laboratory, assay, and storage time', Urology, vol. 45, no. 3, pp. 447-453. https://doi.org/10.1016/S0090-4295(99)80014-4
Jacobsen, Steven J. ; Klee, George G. ; Lilja, Hans ; Wright, George L. ; Oesterling, Joseph E. / Stability of serum prostate-specific antigen determination across laboratory, assay, and storage time. In: Urology. 1995 ; Vol. 45, No. 3. pp. 447-453.
@article{d663ff05802b4325ad7e4fa91f9ba5e2,
title = "Stability of serum prostate-specific antigen determination across laboratory, assay, and storage time",
abstract = "Objectives: To understand the comparability of serum prostate-specific antigen (PSA) determinations across assays and storage time. Methods: Serum PSA levels were determined for men aged 40 to 79 years from the clinical subset of the Olmsted County Study of Urinary Symptoms and Health Status Among Men on fresh samples and after a median of 32 months on banked samples, frozen at -70 °C. Baseline serum PSA levels were determined by Tandem-R PSA assay. Follow-up levels on the banked samples were determined by the IMx PSA assay and a repeat Tandem-R PSA assay in a different laboratory and by an immunofluorometric PSA assay at another site. Results: The median serum PSA level determined by Tandem-R assay at baseline was 1.0 ng/mL (25th percentile, 0.6; 75th percentile, 1.7). The distributions of determination made by follow-up Tandem-R, IMx, and immunofluorometric analyses were essentially identical. Overall, the assays were highly correlated. The correlations between the baseline serum PSA determination and repeated Tandem-R, IMx, and immunofluorometric determinations were 0.96, 0.96, and 0.97, respectively (all P < 0.001). The median duration of frozen storage was 32 months (range, 26 to 39 months), and the correlations between baseline and follow-up determinations did not change when stratified by duration of storage. Conclusions: These data provide important reassurance about the use of serum PSA determinations obtained by different assays, in different laboratories, and in properly tored samples across time.",
author = "Jacobsen, {Steven J.} and Klee, {George G.} and Hans Lilja and Wright, {George L.} and Oesterling, {Joseph E.}",
year = "1995",
doi = "10.1016/S0090-4295(99)80014-4",
language = "English (US)",
volume = "45",
pages = "447--453",
journal = "Urology",
issn = "0090-4295",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Stability of serum prostate-specific antigen determination across laboratory, assay, and storage time

AU - Jacobsen, Steven J.

AU - Klee, George G.

AU - Lilja, Hans

AU - Wright, George L.

AU - Oesterling, Joseph E.

PY - 1995

Y1 - 1995

N2 - Objectives: To understand the comparability of serum prostate-specific antigen (PSA) determinations across assays and storage time. Methods: Serum PSA levels were determined for men aged 40 to 79 years from the clinical subset of the Olmsted County Study of Urinary Symptoms and Health Status Among Men on fresh samples and after a median of 32 months on banked samples, frozen at -70 °C. Baseline serum PSA levels were determined by Tandem-R PSA assay. Follow-up levels on the banked samples were determined by the IMx PSA assay and a repeat Tandem-R PSA assay in a different laboratory and by an immunofluorometric PSA assay at another site. Results: The median serum PSA level determined by Tandem-R assay at baseline was 1.0 ng/mL (25th percentile, 0.6; 75th percentile, 1.7). The distributions of determination made by follow-up Tandem-R, IMx, and immunofluorometric analyses were essentially identical. Overall, the assays were highly correlated. The correlations between the baseline serum PSA determination and repeated Tandem-R, IMx, and immunofluorometric determinations were 0.96, 0.96, and 0.97, respectively (all P < 0.001). The median duration of frozen storage was 32 months (range, 26 to 39 months), and the correlations between baseline and follow-up determinations did not change when stratified by duration of storage. Conclusions: These data provide important reassurance about the use of serum PSA determinations obtained by different assays, in different laboratories, and in properly tored samples across time.

AB - Objectives: To understand the comparability of serum prostate-specific antigen (PSA) determinations across assays and storage time. Methods: Serum PSA levels were determined for men aged 40 to 79 years from the clinical subset of the Olmsted County Study of Urinary Symptoms and Health Status Among Men on fresh samples and after a median of 32 months on banked samples, frozen at -70 °C. Baseline serum PSA levels were determined by Tandem-R PSA assay. Follow-up levels on the banked samples were determined by the IMx PSA assay and a repeat Tandem-R PSA assay in a different laboratory and by an immunofluorometric PSA assay at another site. Results: The median serum PSA level determined by Tandem-R assay at baseline was 1.0 ng/mL (25th percentile, 0.6; 75th percentile, 1.7). The distributions of determination made by follow-up Tandem-R, IMx, and immunofluorometric analyses were essentially identical. Overall, the assays were highly correlated. The correlations between the baseline serum PSA determination and repeated Tandem-R, IMx, and immunofluorometric determinations were 0.96, 0.96, and 0.97, respectively (all P < 0.001). The median duration of frozen storage was 32 months (range, 26 to 39 months), and the correlations between baseline and follow-up determinations did not change when stratified by duration of storage. Conclusions: These data provide important reassurance about the use of serum PSA determinations obtained by different assays, in different laboratories, and in properly tored samples across time.

UR - http://www.scopus.com/inward/record.url?scp=0028892407&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028892407&partnerID=8YFLogxK

U2 - 10.1016/S0090-4295(99)80014-4

DO - 10.1016/S0090-4295(99)80014-4

M3 - Article

C2 - 7533456

AN - SCOPUS:0028892407

VL - 45

SP - 447

EP - 453

JO - Urology

JF - Urology

SN - 0090-4295

IS - 3

ER -