TY - JOUR
T1 - Spliceosome structure
T2 - Piece by piece
AU - Ritchie, Dustin B.
AU - Schellenberg, Matthew J.
AU - MacMillan, Andrew M.
N1 - Funding Information:
We would like to thank Kiyoshi Nagai for sharing results in advance of publication and for assistance with figures. A.M.M. is supported by grants from the Natural Sciences and Engineering Research Council of Canada (NSERC), the Canadian Institutes of Health Research (CIHR), the Alberta Heritage Foundation for Medical Research (AHFMR).
PY - 2009/9
Y1 - 2009/9
N2 - Processing of pre-mRNAs by RNA splicing is an essential step in the maturation of protein coding RNAs in eukaryotes. Structural studies of the cellular splicing machinery, the spliceosome, are a major challenge in structural biology due to the size and complexity of the splicing ensemble. Specifically, the structural details of splice site recognition and the architecture of the spliceosome active site are poorly understood. X-ray and NMR techniques have been successfully used to address these questions defining the structure of individual domains, isolated splicing proteins, spliceosomal RNA fragments and recently the U1 snRNP multiprotein·RNA complex. These results combined with extant biochemical and genetic data have yielded important insights as well as posing fresh questions with respect to the regulation and mechanism of this critical gene regulatory process.
AB - Processing of pre-mRNAs by RNA splicing is an essential step in the maturation of protein coding RNAs in eukaryotes. Structural studies of the cellular splicing machinery, the spliceosome, are a major challenge in structural biology due to the size and complexity of the splicing ensemble. Specifically, the structural details of splice site recognition and the architecture of the spliceosome active site are poorly understood. X-ray and NMR techniques have been successfully used to address these questions defining the structure of individual domains, isolated splicing proteins, spliceosomal RNA fragments and recently the U1 snRNP multiprotein·RNA complex. These results combined with extant biochemical and genetic data have yielded important insights as well as posing fresh questions with respect to the regulation and mechanism of this critical gene regulatory process.
KW - EM
KW - NMR
KW - Spliceosome
KW - Structure
KW - X-ray
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U2 - 10.1016/j.bbagrm.2009.08.010
DO - 10.1016/j.bbagrm.2009.08.010
M3 - Review article
C2 - 19733268
AN - SCOPUS:71849117416
SN - 1874-9399
VL - 1789
SP - 624
EP - 633
JO - Biochimica et Biophysica Acta - Gene Regulatory Mechanisms
JF - Biochimica et Biophysica Acta - Gene Regulatory Mechanisms
IS - 9-10
ER -