Spliceosome structure: Piece by piece

Dustin B. Ritchie, Matthew J. Schellenberg, Andrew M. MacMillan

Research output: Contribution to journalReview articlepeer-review

29 Scopus citations

Abstract

Processing of pre-mRNAs by RNA splicing is an essential step in the maturation of protein coding RNAs in eukaryotes. Structural studies of the cellular splicing machinery, the spliceosome, are a major challenge in structural biology due to the size and complexity of the splicing ensemble. Specifically, the structural details of splice site recognition and the architecture of the spliceosome active site are poorly understood. X-ray and NMR techniques have been successfully used to address these questions defining the structure of individual domains, isolated splicing proteins, spliceosomal RNA fragments and recently the U1 snRNP multiprotein·RNA complex. These results combined with extant biochemical and genetic data have yielded important insights as well as posing fresh questions with respect to the regulation and mechanism of this critical gene regulatory process.

Original languageEnglish (US)
Pages (from-to)624-633
Number of pages10
JournalBiochimica et Biophysica Acta - Gene Regulatory Mechanisms
Volume1789
Issue number9-10
DOIs
StatePublished - Sep 2009

Keywords

  • EM
  • NMR
  • Spliceosome
  • Structure
  • X-ray

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics

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