Spillover of fatty acids during dietary fat storage in type 2 diabetes: Relationship to body fat depots and effects of weight loss

Jaime P. Almandoz, Ekta Kapoor, Lisa A. Howell, Karen Grothe, Danielle T. Vlazny, Almira Smailovic, Brian A. Irving, Robert H. Nelson, John M. Miles

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Spillover of lipoprotein lipase-generated fatty acids from chylomicrons into the plasma free fatty acid (FFA) pool is an important source of FFA and reflects inefficiency in dietary fat storage. We measured spillover in 13 people with type 2 diabetes using infusions of a [3H]triolein-labeled lipid emulsion and [U-13C]oleate during continuous feeding, before and after weight loss. Body fat was measured with dual energy X-ray absorptiometry and computed tomography. Participants lost ∼14% of body weight. There was an ∼38% decrease in meal-suppressed FFA concentration (P < 0.0001) and an ∼23% decrease in oleate flux (P = 0.007). Fractional spillover did not change (P = NS). At baseline, there was a strong negative correlation between spillover and leg fat (r = -0.79, P = 0.001) and a positive correlation with the trunk-to-leg fat ratio (R = 0.56, P = 0.047). These correlations disappeared after weight loss. Baseline leg fat (R = 20.61, P = 0.027) but not trunk fat (R = 20.27, P = 0.38) negatively predicted decreases in spillover with weight loss. These results indicate that spillover, a measure of inefficiency in dietary fat storage, is inversely associated with lower body fat in type 2 diabetes.

Original languageEnglish (US)
Pages (from-to)1897-1903
Number of pages7
JournalDiabetes
Volume62
Issue number6
DOIs
StatePublished - Jun 2013

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Dietary Fats
Type 2 Diabetes Mellitus
Adipose Tissue
Weight Loss
Fatty Acids
Fats
Nonesterified Fatty Acids
Leg
Oleic Acid
Triolein
Chylomicrons
X Ray Computed Tomography
Lipoprotein Lipase
Photon Absorptiometry
Emulsions
Meals
Body Weight
Lipids

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Spillover of fatty acids during dietary fat storage in type 2 diabetes : Relationship to body fat depots and effects of weight loss. / Almandoz, Jaime P.; Kapoor, Ekta; Howell, Lisa A.; Grothe, Karen; Vlazny, Danielle T.; Smailovic, Almira; Irving, Brian A.; Nelson, Robert H.; Miles, John M.

In: Diabetes, Vol. 62, No. 6, 06.2013, p. 1897-1903.

Research output: Contribution to journalArticle

Almandoz, JP, Kapoor, E, Howell, LA, Grothe, K, Vlazny, DT, Smailovic, A, Irving, BA, Nelson, RH & Miles, JM 2013, 'Spillover of fatty acids during dietary fat storage in type 2 diabetes: Relationship to body fat depots and effects of weight loss', Diabetes, vol. 62, no. 6, pp. 1897-1903. https://doi.org/10.2337/db12-1407
Almandoz, Jaime P. ; Kapoor, Ekta ; Howell, Lisa A. ; Grothe, Karen ; Vlazny, Danielle T. ; Smailovic, Almira ; Irving, Brian A. ; Nelson, Robert H. ; Miles, John M. / Spillover of fatty acids during dietary fat storage in type 2 diabetes : Relationship to body fat depots and effects of weight loss. In: Diabetes. 2013 ; Vol. 62, No. 6. pp. 1897-1903.
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