Species characterization of plasma nitrite/nitrate (NOx) concentration

Donna A. Williams, Debra A. Lewis, William J. Krause, Mary H. Flood, Virginia M Miller

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Experiments were designed to detect and determine differences between nitrite/nitrate concentration ([NOx]) in plasma across 15 species selected from seven classes of vertebrates. Blood collected in syringes was placed immediately into ethylenediaminetetraacetic acid (EDTA)-containing tubes and was centrifuged. Plasma [NOx] was determined by measurement of chemiluminescence. Across classes of vertebrates, baseline plasma [NOx] ranged from 0.6 to 171.3 nmol/ml. Mean ± SD plasma [NOx] was highest in a fresh-water, jawless fish (lamprey, 95.5 ± 9.1 nmol/ml) and lowest in a saltwater cartilaginous fish (skates, 1.1 ± 0.4 nmol/ml). Both amphibians tested had a wide range in plasma [NOx], which was explained partly by temporal changes during the year. Within the mammalian class, plasma [NOx] ranged from 3.8 to 43.2 nmol/ml. Results of this study indicate that NOx is detectable in plasma of all classes of vertebrates and that baseline concentration varies among species.

Original languageEnglish (US)
Pages (from-to)21-28
Number of pages8
JournalComparative Medicine
Volume53
Issue number1
StatePublished - Feb 2003

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Nitrites
Nitrates
nitrites
vertebrates
nitrates
Plasmas
Rajidae
Chondrichthyes
Petromyzontiformes
chemiluminescence
syringes
Vertebrates
EDTA (chelating agent)
saline water
freshwater fish
amphibians
Fish
Skates (Fish)
Luminescent Measurements
Lampreys

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Williams, D. A., Lewis, D. A., Krause, W. J., Flood, M. H., & Miller, V. M. (2003). Species characterization of plasma nitrite/nitrate (NOx) concentration. Comparative Medicine, 53(1), 21-28.

Species characterization of plasma nitrite/nitrate (NOx) concentration. / Williams, Donna A.; Lewis, Debra A.; Krause, William J.; Flood, Mary H.; Miller, Virginia M.

In: Comparative Medicine, Vol. 53, No. 1, 02.2003, p. 21-28.

Research output: Contribution to journalArticle

Williams, DA, Lewis, DA, Krause, WJ, Flood, MH & Miller, VM 2003, 'Species characterization of plasma nitrite/nitrate (NOx) concentration', Comparative Medicine, vol. 53, no. 1, pp. 21-28.
Williams DA, Lewis DA, Krause WJ, Flood MH, Miller VM. Species characterization of plasma nitrite/nitrate (NOx) concentration. Comparative Medicine. 2003 Feb;53(1):21-28.
Williams, Donna A. ; Lewis, Debra A. ; Krause, William J. ; Flood, Mary H. ; Miller, Virginia M. / Species characterization of plasma nitrite/nitrate (NOx) concentration. In: Comparative Medicine. 2003 ; Vol. 53, No. 1. pp. 21-28.
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