Somatomedin C-binding and action in fibroblasts from aged and progeric subjects

Cheryl A Conover, L. A. Dollar, R. G. Rosenfeld, R. L. Hinz

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Aging is associated with diminished cell growth, which has been ascribed in part to decreased cellular responsiveness to serum mitogens. To investigate whether there is an age-related loss of responsiveness to somatomedin-C (SM-C), we studied SM-C binding and action in early passage fibroblasts from normal donors, aged 7-96 yr, and one progeric subject. SM-C stimulated [3H]thymidine incorporation 4- to 16-fold in young cells, 4- to 17-fold in aged cells, and 4- to 11-fold in progeric cells. SM-C was synergistic with 0.25% human hypopituitary serum in stimulating [3H]thymidine incorporation in all cell lines. Dose-response curves for SM-C stimulation of thymidine incorporation were not significantly altered in aged or progeric cells. Half-maximal responses occurred at 5-15 ng/ml SM-C for all cell lines. [3H]Thymidine incorporation results were supported by cell replication studies. In addition, binding of [125I] SM-C was virtually identical in all cell lines, with 50% displacement at 2-5 ng/ml SM-C. Thus, in vivo aging does not appear to be associated with either an alteration in SM-C receptors or a diminished cellular responsiveness to SM-C's mitogenic effects.

Original languageEnglish (US)
Pages (from-to)685-691
Number of pages7
JournalJournal of Clinical Endocrinology and Metabolism
Volume60
Issue number4
StatePublished - 1985
Externally publishedYes

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Fibroblasts
Insulin-Like Growth Factor I
Thymidine
Cells
Cell Line
Aging of materials
Somatomedin Receptors
Cell growth
Serum
Mitogens
Growth

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Somatomedin C-binding and action in fibroblasts from aged and progeric subjects. / Conover, Cheryl A; Dollar, L. A.; Rosenfeld, R. G.; Hinz, R. L.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 60, No. 4, 1985, p. 685-691.

Research output: Contribution to journalArticle

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