Soluble Interleukin-13Rα2 Decoy Receptor Inhibits Hodgkin's Lymphoma Growth in Vitro and in Vivo

Young Trieu, Xiao Yan Wen, Brian F. Skinnider, Mark R. Bray, Zhihua Li, Jaime O. Claudio, Esther Masih-Khan, Yuan Xiao Zhu, Suzanne Trudel, J. Andrea McCart, Tak W. Mak, A. Keith Stewart

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Recent studies have demonstrated that the malignant Reed-Sternberg cells of Hodgkin's lymphoma (HL) secrete and are responsive to interleukin (IL)-13. We hypothesized that overexpression of a soluble IL-13 decoy receptor (sIL-13Rα2) via adenoviral-mediated gene transfer would inhibit IL-13-induced Reed-Sternberg cell proliferation. Western blot and ELISA analysis verified expression of sIL-13Rα2 in cell lysates and supernatants of AdsIL-13Rα2-transduced COS-7 cells. Treatment of two IL-13-responsive HL-derived cell lines, HDLM-2 and L-1236, with AdsIL-13Rα2-conditioned medium, resulted in the inhibition of cell proliferation, and down-regulated the phosphorylation of signal transducer and activator of transcription 6 (STAT6), an important mediator of IL-13 signaling. i.v. delivery of AdsIL-13Rα2 in NOD/SCID mice with s.c. implanted HDLM-2 cells delayed tumor onset and growth while enhancing survival compared with control mice. Intratumoral administration of AdsIL-13Rα2 led to the regression or stabilization of established tumors and was associated with diminished STAT6 phosphorylation. Our data demonstrate that AdsIL-13Rα2 can suppress HL growth in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)3271-3275
Number of pages5
JournalCancer research
Volume64
Issue number9
DOIs
StatePublished - May 1 2004

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Trieu, Y., Wen, X. Y., Skinnider, B. F., Bray, M. R., Li, Z., Claudio, J. O., Masih-Khan, E., Zhu, Y. X., Trudel, S., McCart, J. A., Mak, T. W., & Stewart, A. K. (2004). Soluble Interleukin-13Rα2 Decoy Receptor Inhibits Hodgkin's Lymphoma Growth in Vitro and in Vivo. Cancer research, 64(9), 3271-3275. https://doi.org/10.1158/0008-5472.CAN-03-3764