SNX17 affects T cell activation by regulating TCR and integrin recycling

Douglas G. Osborne, Joshua T. Piotrowski, Christopher J. Dick, Jin San Zhang, Daniel D Billadeau

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

A key component in T cell activation is the endosomal recycling of receptors to the cell surface, thereby allowing continual integration of signaling and Ag recognition. One protein potentially involved in TCR transport is sorting nexin 17 (SNX17). SNX proteins have been found to bind proteins involved in T cell activation, but specifically the role of SNX17 in receptor recycling and T cell activation is unknown. Using immunofluorescence, we find that SNX17 colocalizes with TCR and localizes to the immune synapse in T-conjugates. Significantly, knockdown of the SNX17 resulted in fewer T-APC conjugates, lower CD69, TCR, and LFA-1 surface expression, as well as lower overall TCR recycling compared with control T cells. Lastly, we identified the 4.1/ezrin/radixin/moesin domain of SNX17 as being responsible in the binding and trafficking of TCR and LFA-1 to the cell surface. These data suggest that SNX17 plays a role in the maintenance of normal surface levels of activating receptors and integrins to permit optimum T cell activation at the immune synapse.

Original languageEnglish (US)
Pages (from-to)4555-4566
Number of pages12
JournalJournal of Immunology
Volume194
Issue number9
DOIs
StatePublished - May 1 2015

ASJC Scopus subject areas

  • Immunology

Fingerprint Dive into the research topics of 'SNX17 affects T cell activation by regulating TCR and integrin recycling'. Together they form a unique fingerprint.

  • Cite this