Snrk-1 is involved in multiple steps of angioblast development and acts via notch signaling pathway in artery-vein specification in vertebrates

Chang Z. Chun, Sukhbir Kaur, Ganesh V. Samant, Ling Wang, Kallal Pramanik, Maija K. Garnaas, Keguo Li, Lyndsay Field, Debabrata Mukhopadhyay, Ramani Ramchandranl

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

In vertebrates, molecular mechanisms dictate angioblasts' migration and subsequent differentiation into arteries and veins. In this study, we used a microarray screen to identify a novel member of the sucrose nonfermenting related kinase (snrk-1) family of serine/threonine kinases expressed specifically in the embryonic zebrafish vasculature and investigated its function in vivo. Using gain-and loss-of-function studies in vivo, we show that Snrk-1 plays an essential role in the migration, maintenance, and differentiation of angioblasts. The kinase function of Snrk-1 is critical for migration and maintenance, but not for the differentiation of angioblasts. In vitro, snrk-1 knockdown endothelial cells show only defects in migration. The snrk-1 gene acts downstream or parallel to notch and upstream of gridlock during artery-vein specification, and the human gene compensates for zebrafish snrk-1 knockdown, suggesting evolutionary conservation of function.

Original languageEnglish (US)
Pages (from-to)1192-1199
Number of pages8
JournalBlood
Volume113
Issue number5
DOIs
StatePublished - Jan 29 2009

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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    Chun, C. Z., Kaur, S., Samant, G. V., Wang, L., Pramanik, K., Garnaas, M. K., Li, K., Field, L., Mukhopadhyay, D., & Ramchandranl, R. (2009). Snrk-1 is involved in multiple steps of angioblast development and acts via notch signaling pathway in artery-vein specification in vertebrates. Blood, 113(5), 1192-1199. https://doi.org/10.1182/blood-2008-06-162156