Ski is a component of the histone deacetylase complex required for transcriptional repression by Mad and thyroid hormone receptor

Teruaki Nomura, Md Matiullah Khan, Sunil C. Kaul, Haidong M Dong, Renu Wadhwa, Clemencia Colmenares, Isao Kohno, Shunsuke Ishii

Research output: Contribution to journalArticle

233 Citations (Scopus)

Abstract

The N-CoR/SMRT complex containing mSin3 and histone deacetylase (HDAC) mediates transcriptional repression by nuclear hormone receptors and Mad. The proteins encoded by the ski proto-oncogene family directly bind to N-CoR/SMRT and mSin3A, and forms a complex with HDAC. c-Ski and its related gene product Sno are required for transcriptional repression by Mad and thyroid hormone receptor (TRβ). The oncogenic form, v-Ski, which lacks the mSin3A-binding domain, acts in a dominant-negative fashion, and abrogates transcriptional repression by Mad and TRβ. In ski-deficient mouse embryos, the ornithine decarboxylase gene, whose expression is normally repressed by Mad-Max, is expressed ectopically. These results show that Ski is a component of the HDAC complex and that Ski is required for the transcriptional repression mediated by this complex. The involvement of c-Ski in the HDAC complex indicates that the function of the HDAC complex is important for oncogenesis.

Original languageEnglish (US)
Pages (from-to)412-423
Number of pages12
JournalGenes and Development
Volume13
Issue number4
StatePublished - Feb 15 1999
Externally publishedYes

Fingerprint

Thyroid Hormone Receptors
Histone Deacetylases
Ornithine Decarboxylase
Proto-Oncogenes
Cytoplasmic and Nuclear Receptors
Carcinogenesis
Embryonic Structures
Gene Expression
Genes
Proteins

Keywords

  • Histone deacetylase complex
  • Mad
  • mSin3
  • N-CoR/SMRT corepressor
  • Ski

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Ski is a component of the histone deacetylase complex required for transcriptional repression by Mad and thyroid hormone receptor. / Nomura, Teruaki; Khan, Md Matiullah; Kaul, Sunil C.; Dong, Haidong M; Wadhwa, Renu; Colmenares, Clemencia; Kohno, Isao; Ishii, Shunsuke.

In: Genes and Development, Vol. 13, No. 4, 15.02.1999, p. 412-423.

Research output: Contribution to journalArticle

Nomura, T, Khan, MM, Kaul, SC, Dong, HM, Wadhwa, R, Colmenares, C, Kohno, I & Ishii, S 1999, 'Ski is a component of the histone deacetylase complex required for transcriptional repression by Mad and thyroid hormone receptor', Genes and Development, vol. 13, no. 4, pp. 412-423.
Nomura, Teruaki ; Khan, Md Matiullah ; Kaul, Sunil C. ; Dong, Haidong M ; Wadhwa, Renu ; Colmenares, Clemencia ; Kohno, Isao ; Ishii, Shunsuke. / Ski is a component of the histone deacetylase complex required for transcriptional repression by Mad and thyroid hormone receptor. In: Genes and Development. 1999 ; Vol. 13, No. 4. pp. 412-423.
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