Signalling protein complexes isolated from primary human skin-resident T cells can be analysed by Multiplex IP-FCM

Stephen E.P. Smith, Steven C. Neier, Tessa R. Davis, Mark R. Pittelkow, Diana Gil, Adam G. Schrum

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Studying signal transduction in skin-resident T cells (sr-T cells) can be limited by the small size of clinical biopsies. Here, we isolated sr-T cells from clinical samples and analysed signalling protein complexes by multiplex immunoprecipitation detected by flow cytometry (mIP-FCM). In samples from two independent donors, antigenic stimulation induced signalling proteins to join shared complexes that were observed in seven pairwise combinations among five proteins. This demonstrates that sr-T cells isolated from small clinical samples provide sufficient material for mIP-FCM-based analysis of signalling-induced protein complexes. We propose that this strategy may be useful for gaining improved mechanistic insight of sr-T cell signal transduction associated with dermatological disease.

Original languageEnglish (US)
Pages (from-to)272-273
Number of pages2
JournalExperimental Dermatology
Volume23
Issue number4
DOIs
StatePublished - Apr 2014

Keywords

  • Multiplex technology
  • Protein-protein interaction
  • Signal transduction
  • Skin-resident T cell
  • T cell antigen receptor

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

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    Smith, S. E. P., Neier, S. C., Davis, T. R., Pittelkow, M. R., Gil, D., & Schrum, A. G. (2014). Signalling protein complexes isolated from primary human skin-resident T cells can be analysed by Multiplex IP-FCM. Experimental Dermatology, 23(4), 272-273. https://doi.org/10.1111/exd.12362