Side chain oxidation of 1,25-dihydroxyvitamin D3 in the rat: Effect of removal of the intestine

Rajiv Kumar, H. F. DeLuca

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Removal of the jejunum, ileum and colon in the rat reduces the amount of 14CO2 formed after an intravenous injection of 325 pmoles of 1,25-dihydroxy-[26,27-14C]vitamin D3, by 65.2 ± 13.2% at 4 hours and 67.1 ± 9.12% at 8 hours. This suggests that the intestine may be one of the sites where side chain oxidation occurs. It is possible that the liver may also be involved in this process as removal of a large portion of the gut may disturb hepatic metabolism secondary to a reduction in portal blood flow. The process is not bacterial inasmuch as "germ free" animals produce at least as much 14CO2 after the administration of 1,25-dihydroxy-[26,27-14C]vitamin D3 as do non-germ free controls.

Original languageEnglish (US)
Pages (from-to)253-258
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume76
Issue number2
DOIs
StatePublished - May 23 1977
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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