Side chain oxidation of 1,25-dihydroxyvitamin D3 in the rat: Effect of removal of the intestine

Rajiv Kumar; H. F. DeLuca

doi:10.1016/0006-291X(77)90719-7

Side chain oxidation of 1,25-dihydroxyvitamin D₃ in the rat: Effect of removal of the intestine

Rajiv Kumar, H. F. DeLuca

Nephrology and Hypertension

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Removal of the jejunum, ileum and colon in the rat reduces the amount of ¹⁴CO₂ formed after an intravenous injection of 325 pmoles of 1,25-dihydroxy-[26,27-¹⁴C]vitamin D₃, by 65.2 ± 13.2% at 4 hours and 67.1 ± 9.12% at 8 hours. This suggests that the intestine may be one of the sites where side chain oxidation occurs. It is possible that the liver may also be involved in this process as removal of a large portion of the gut may disturb hepatic metabolism secondary to a reduction in portal blood flow. The process is not bacterial inasmuch as "germ free" animals produce at least as much ¹⁴CO₂ after the administration of 1,25-dihydroxy-[26,27-¹⁴C]vitamin D₃ as do non-germ free controls.

Original language	English (US)
Pages (from-to)	253-258
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	76
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(77)90719-7
State	Published - May 23 1977

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(77)90719-7

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title = "Side chain oxidation of 1,25-dihydroxyvitamin D3 in the rat: Effect of removal of the intestine",

abstract = "Removal of the jejunum, ileum and colon in the rat reduces the amount of 14CO2 formed after an intravenous injection of 325 pmoles of 1,25-dihydroxy-[26,27-14C]vitamin D3, by 65.2 ± 13.2% at 4 hours and 67.1 ± 9.12% at 8 hours. This suggests that the intestine may be one of the sites where side chain oxidation occurs. It is possible that the liver may also be involved in this process as removal of a large portion of the gut may disturb hepatic metabolism secondary to a reduction in portal blood flow. The process is not bacterial inasmuch as {"}germ free{"} animals produce at least as much 14CO2 after the administration of 1,25-dihydroxy-[26,27-14C]vitamin D3 as do non-germ free controls.",

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T1 - Side chain oxidation of 1,25-dihydroxyvitamin D3 in the rat

T2 - Effect of removal of the intestine

AU - Kumar, Rajiv

AU - DeLuca, H. F.

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N2 - Removal of the jejunum, ileum and colon in the rat reduces the amount of 14CO2 formed after an intravenous injection of 325 pmoles of 1,25-dihydroxy-[26,27-14C]vitamin D3, by 65.2 ± 13.2% at 4 hours and 67.1 ± 9.12% at 8 hours. This suggests that the intestine may be one of the sites where side chain oxidation occurs. It is possible that the liver may also be involved in this process as removal of a large portion of the gut may disturb hepatic metabolism secondary to a reduction in portal blood flow. The process is not bacterial inasmuch as "germ free" animals produce at least as much 14CO2 after the administration of 1,25-dihydroxy-[26,27-14C]vitamin D3 as do non-germ free controls.

AB - Removal of the jejunum, ileum and colon in the rat reduces the amount of 14CO2 formed after an intravenous injection of 325 pmoles of 1,25-dihydroxy-[26,27-14C]vitamin D3, by 65.2 ± 13.2% at 4 hours and 67.1 ± 9.12% at 8 hours. This suggests that the intestine may be one of the sites where side chain oxidation occurs. It is possible that the liver may also be involved in this process as removal of a large portion of the gut may disturb hepatic metabolism secondary to a reduction in portal blood flow. The process is not bacterial inasmuch as "germ free" animals produce at least as much 14CO2 after the administration of 1,25-dihydroxy-[26,27-14C]vitamin D3 as do non-germ free controls.

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Side chain oxidation of 1,25-dihydroxyvitamin D₃ in the rat: Effect of removal of the intestine

Abstract

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