Sex-specific changes in platelet aggregation and secretion with sexual maturity in pigs

Muthuvel Jayachandran, Hiroya Okano, Ritu Chatrath, Whyte G. Owen, Joseph P. McConnell, Virginia M Miller

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Cardiovascular disease may begin early in adolescence. Platelets release factors contributing to vascular disease. Experiments were designed to test the hypothesis that hormonal transitions associated with sexual maturity differentially affect platelet aggregation and secretion in males and females. Platelets were collected from juvenile (2-3 mo) and sexually mature (adult; 5-6 mo) male and female pigs (n = 8/group). Maturation was evidenced by increased weight of reproductive tissue and changes in circulating levels of gonadal hormones. Aggregation to ADP (10 μM) and collagen (6 μg/ml) and ATP secretion to 50 nM thrombin were determined by turbidimetric analysis and bioluminescence, respectively. Total platelet counts, platelet turnover, and mean platelet volume did not change with maturity. Platelet aggregation and ATP secretion decreased in females but increased in males with maturity, whereas total ATP content remained unchanged in platelets from females but increased in platelets from males. Platelet fibrinogen receptor, P-selectin expression, and receptors for sex steroids did not change with sexual maturation. Plasma C-reactive protein and brain-type natriuretic peptide also did not change. Results indicate that changes in platelet aggregation and secretion change with sexual maturity differently in females and males. These observations provide evidence on which clinical studies could be designed to examine platelet characteristics in human children and young adults.

Original languageEnglish (US)
Pages (from-to)1445-1452
Number of pages8
JournalJournal of Applied Physiology
Volume97
Issue number4
DOIs
StatePublished - Oct 2004

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Platelet Aggregation
Swine
Blood Platelets
Adenosine Triphosphate
Fibrinogen Receptors
Mean Platelet Volume
Gonadal Hormones
Sexual Maturation
P-Selectin
Steroid Receptors
Brain Natriuretic Peptide
Platelet Count
Vascular Diseases
Thrombin
C-Reactive Protein
Adenosine Diphosphate
Blood Proteins
Young Adult
Cardiovascular Diseases
Collagen

Keywords

  • Estrogen
  • Platelet activation
  • Sex steroid receptors
  • Testosterone

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Sex-specific changes in platelet aggregation and secretion with sexual maturity in pigs. / Jayachandran, Muthuvel; Okano, Hiroya; Chatrath, Ritu; Owen, Whyte G.; McConnell, Joseph P.; Miller, Virginia M.

In: Journal of Applied Physiology, Vol. 97, No. 4, 10.2004, p. 1445-1452.

Research output: Contribution to journalArticle

Jayachandran, Muthuvel ; Okano, Hiroya ; Chatrath, Ritu ; Owen, Whyte G. ; McConnell, Joseph P. ; Miller, Virginia M. / Sex-specific changes in platelet aggregation and secretion with sexual maturity in pigs. In: Journal of Applied Physiology. 2004 ; Vol. 97, No. 4. pp. 1445-1452.
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