Sex and depot differences in ex vivo adipose tissue fatty acid storage and glycerol-3-phosphate acyltransferase activity

Maria Morgan-Bathke, Liang Chen, Elisabeth Oberschneider, Debra Harteneck, Michael Dennis Jensen

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Adipose tissue fatty acid storage varies according to sex, adipose tissue depot, and degree of fat gain. However, the mechanism(s) for these variations is not completely understood. We examined whether differences in adipose tissue glycerol-3-phosphate acyltransferase (GPAT) might play a role in these variations. We optimized an enzyme activity assay for total GPAT and GPAT1 activity in human adipose tissue and measured GPAT activity. Omental and subcutaneous adipose tissue was collected from obese and nonobese adults for measures of GPAT and GPAT1 activities, ex vivo palmitate storage, acyl-CoA synthetase (ACS) and diacylglycerol-acyltransferase (DGAT) activities, and CD36 protein. Total GPAT and GPAT1 activities decreased as a function of adipocyte size in both omental (r=-0.71, P = 0.003) and subcutaneous (r=-0.58, P = 0.04) fat. The relative contribution of GPAT1 to total GPAT activity increased as a function of adipocyte size, accounting for up to 60% of GPAT activity in those with the largest adipocytes. We found strong, positive correlations between ACS, GPAT, and DGAT activities for both sexes and depots (r values 0.58–0.91) and between these storage factors and palmitate storage rates into TAG (r values 0.55– 0.90). We conclude that: 1) total GPAT activity decreases as a function of adipocyte size; 2) GPAT1 can account for over half of adipose GPAT activity in hypertrophic obesity; and 3) ACS, GPAT, and DGAT are coordinately regulated.

Original languageEnglish (US)
Pages (from-to)E830-E846
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume308
Issue number9
DOIs
StatePublished - 2015

Fingerprint

Acyltransferases
Sex Characteristics
Adipose Tissue
Fatty Acids
Diacylglycerol O-Acyltransferase
Coenzyme A Ligases
Adipocytes
Palmitates
alpha-glycerophosphoric acid
Fats
CD36 Antigens
Subcutaneous Fat
Enzyme Assays
Human Activities
Obesity

Keywords

  • Fat distribution
  • Glycerol-3-phosphate acyltransferase 1
  • Omental fat
  • Subcutaneous fat

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Endocrinology, Diabetes and Metabolism

Cite this

Sex and depot differences in ex vivo adipose tissue fatty acid storage and glycerol-3-phosphate acyltransferase activity. / Morgan-Bathke, Maria; Chen, Liang; Oberschneider, Elisabeth; Harteneck, Debra; Jensen, Michael Dennis.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 308, No. 9, 2015, p. E830-E846.

Research output: Contribution to journalArticle

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