Serum lipoproteins and albumin in the lecithin: Cholesterol acyltransferase reaction

Jeffrey K. Yao; Steve C.S. Chang; Robert J. Ryan; Peter James Dyck

doi:10.1016/0006-291X(80)90848-7

Serum lipoproteins and albumin in the lecithin: Cholesterol acyltransferase reaction

Jeffrey K. Yao, Steve C.S. Chang, Robert J. Ryan, Peter James Dyck

Neurology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The unique features of pig ovarian follicular fluids, i.e., presence of high density lipoprotein (HDL) only and lecithin: cholesterol acyltransferase (EC 2.3.1.43; LCAT) activity, provides a good model to study the effect of serum lipoproteins and serum albumin on the LCAT reaction. Invitro cholesterol esterification is enhanced when very low density lipoprotein (VLDL) and low density lipoprotein (LDL) fractions are added, but is inhibited when one or the other of these lipoproteins is absent. High concentrations of HDL₂ result in decreased activation which can be compensated for by the addition of the VLDL-LDL mixture. These findings suggest that the rate of cholesterol esterification in ovarian follicular fluid may be enhanced by providing the exogenous VLDL and LDL as the recipients of HDL-cholesteryl ester. The inhibition of LCAT activity caused by free fatty acid and lysophosphatidylcholine can be partially reversed by the addition of serum albumin, suggesting that serum albumin may regulate the LCAT reaction.

Original language	English (US)
Pages (from-to)	738-744
Number of pages	7
Journal	Topics in Catalysis
Volume	95
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(80)90848-7
State	Published - 1980

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Biophysics
Molecular Biology

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title = "Serum lipoproteins and albumin in the lecithin: Cholesterol acyltransferase reaction",

abstract = "The unique features of pig ovarian follicular fluids, i.e., presence of high density lipoprotein (HDL) only and lecithin: cholesterol acyltransferase (EC 2.3.1.43; LCAT) activity, provides a good model to study the effect of serum lipoproteins and serum albumin on the LCAT reaction. Invitro cholesterol esterification is enhanced when very low density lipoprotein (VLDL) and low density lipoprotein (LDL) fractions are added, but is inhibited when one or the other of these lipoproteins is absent. High concentrations of HDL2 result in decreased activation which can be compensated for by the addition of the VLDL-LDL mixture. These findings suggest that the rate of cholesterol esterification in ovarian follicular fluid may be enhanced by providing the exogenous VLDL and LDL as the recipients of HDL-cholesteryl ester. The inhibition of LCAT activity caused by free fatty acid and lysophosphatidylcholine can be partially reversed by the addition of serum albumin, suggesting that serum albumin may regulate the LCAT reaction.",

author = "Yao, {Jeffrey K.} and Chang, {Steve C.S.} and Ryan, {Robert J.} and Dyck, {Peter James}",

year = "1980",

doi = "10.1016/0006-291X(80)90848-7",

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T1 - Serum lipoproteins and albumin in the lecithin

T2 - Cholesterol acyltransferase reaction

AU - Yao, Jeffrey K.

AU - Chang, Steve C.S.

AU - Ryan, Robert J.

AU - Dyck, Peter James

PY - 1980

Y1 - 1980

N2 - The unique features of pig ovarian follicular fluids, i.e., presence of high density lipoprotein (HDL) only and lecithin: cholesterol acyltransferase (EC 2.3.1.43; LCAT) activity, provides a good model to study the effect of serum lipoproteins and serum albumin on the LCAT reaction. Invitro cholesterol esterification is enhanced when very low density lipoprotein (VLDL) and low density lipoprotein (LDL) fractions are added, but is inhibited when one or the other of these lipoproteins is absent. High concentrations of HDL2 result in decreased activation which can be compensated for by the addition of the VLDL-LDL mixture. These findings suggest that the rate of cholesterol esterification in ovarian follicular fluid may be enhanced by providing the exogenous VLDL and LDL as the recipients of HDL-cholesteryl ester. The inhibition of LCAT activity caused by free fatty acid and lysophosphatidylcholine can be partially reversed by the addition of serum albumin, suggesting that serum albumin may regulate the LCAT reaction.

AB - The unique features of pig ovarian follicular fluids, i.e., presence of high density lipoprotein (HDL) only and lecithin: cholesterol acyltransferase (EC 2.3.1.43; LCAT) activity, provides a good model to study the effect of serum lipoproteins and serum albumin on the LCAT reaction. Invitro cholesterol esterification is enhanced when very low density lipoprotein (VLDL) and low density lipoprotein (LDL) fractions are added, but is inhibited when one or the other of these lipoproteins is absent. High concentrations of HDL2 result in decreased activation which can be compensated for by the addition of the VLDL-LDL mixture. These findings suggest that the rate of cholesterol esterification in ovarian follicular fluid may be enhanced by providing the exogenous VLDL and LDL as the recipients of HDL-cholesteryl ester. The inhibition of LCAT activity caused by free fatty acid and lysophosphatidylcholine can be partially reversed by the addition of serum albumin, suggesting that serum albumin may regulate the LCAT reaction.

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ER -

Serum lipoproteins and albumin in the lecithin: Cholesterol acyltransferase reaction

Abstract

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