Serodiagnosis of Helicobacter pylori: Comparison of enzyme-linked immunosorbent assays

N. J. Talley, D. G. Newell, J. E. Ormand, H. A. Carpenter, W. R. Wilson, A. R. Zinsmeister, G. I. Perez-Perez, M. J. Blaser

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

Enzyme-linked immunosorbent assays (ELISAs) have been developed to diagnose Helicobacter pylori infection. However, the methods are not standardized. We therefore prospectively evaluated the sensitivities and specificities of ELISAs developed in the United States and the United Kingdom in a study population comprising 41 consecutive symptomatic outpatients and 35 volunteers. At endoscopy, multiple biopsies were obtained for histology and culture and stained sections were graded for chronic gastritis, active chronic gastritis, and density of H. pylori. Serum samples were analyzed for H. pylori by ELISA. The first set of assays for immunoglobulin G (IgG) and IgA used a pool of sonicated isolates of H. pylori from five patients in the United States (antigen A). The second set of assays, developed in the United Kingdom, used three different antigens: antigen 1, an acid-extractable surface antigen; antigen 2, an acid-extractable antigen from an aflagellate variant; and antigen 3, a urease-containing fraction. Cutoff scores for positive results were determined a priori on the basis of previous serological studies. There was close agreement between histology and culture. In the study population, 36% of the individuals were H. pylori positive. The diagnostic value of the different ELISAs were highly comparable, and the crude antigens performed as well as the more purified antigens. The antigen A IgG had a sensitivity and specificity of 96 and 94%, respectively; the values for antigen 1 were 93 and 96%, respectively. The antigen A IgA and antigen 3 assays were the least sensitive tests. All of the serological assays were significantly associated with active chronic gastritis scores, but the IgA assay provided additional independent information for discriminating mild active gastritis from more severe gastritis. Despite the antigenic variation that exists between H. pylori strains, the diagnostic characteristics of these ELISAs from the United States and the United Kingdom are similarly highly sensitive and specific.

Original languageEnglish (US)
Pages (from-to)1635-1639
Number of pages5
JournalJournal of Clinical Microbiology
Volume29
Issue number8
StatePublished - 1991

Fingerprint

Serologic Tests
Helicobacter pylori
Enzyme-Linked Immunosorbent Assay
Antigens
Gastritis
Immunoglobulin A
Histology
Immunoglobulin G
Antigenic Variation
Sensitivity and Specificity
Acids
Urease
Helicobacter Infections
Surface Antigens
Population
Endoscopy
Volunteers
Outpatients
Biopsy

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Talley, N. J., Newell, D. G., Ormand, J. E., Carpenter, H. A., Wilson, W. R., Zinsmeister, A. R., ... Blaser, M. J. (1991). Serodiagnosis of Helicobacter pylori: Comparison of enzyme-linked immunosorbent assays. Journal of Clinical Microbiology, 29(8), 1635-1639.

Serodiagnosis of Helicobacter pylori : Comparison of enzyme-linked immunosorbent assays. / Talley, N. J.; Newell, D. G.; Ormand, J. E.; Carpenter, H. A.; Wilson, W. R.; Zinsmeister, A. R.; Perez-Perez, G. I.; Blaser, M. J.

In: Journal of Clinical Microbiology, Vol. 29, No. 8, 1991, p. 1635-1639.

Research output: Contribution to journalArticle

Talley, NJ, Newell, DG, Ormand, JE, Carpenter, HA, Wilson, WR, Zinsmeister, AR, Perez-Perez, GI & Blaser, MJ 1991, 'Serodiagnosis of Helicobacter pylori: Comparison of enzyme-linked immunosorbent assays', Journal of Clinical Microbiology, vol. 29, no. 8, pp. 1635-1639.
Talley NJ, Newell DG, Ormand JE, Carpenter HA, Wilson WR, Zinsmeister AR et al. Serodiagnosis of Helicobacter pylori: Comparison of enzyme-linked immunosorbent assays. Journal of Clinical Microbiology. 1991;29(8):1635-1639.
Talley, N. J. ; Newell, D. G. ; Ormand, J. E. ; Carpenter, H. A. ; Wilson, W. R. ; Zinsmeister, A. R. ; Perez-Perez, G. I. ; Blaser, M. J. / Serodiagnosis of Helicobacter pylori : Comparison of enzyme-linked immunosorbent assays. In: Journal of Clinical Microbiology. 1991 ; Vol. 29, No. 8. pp. 1635-1639.
@article{894847180ea4484982c0e6f7fdd9f576,
title = "Serodiagnosis of Helicobacter pylori: Comparison of enzyme-linked immunosorbent assays",
abstract = "Enzyme-linked immunosorbent assays (ELISAs) have been developed to diagnose Helicobacter pylori infection. However, the methods are not standardized. We therefore prospectively evaluated the sensitivities and specificities of ELISAs developed in the United States and the United Kingdom in a study population comprising 41 consecutive symptomatic outpatients and 35 volunteers. At endoscopy, multiple biopsies were obtained for histology and culture and stained sections were graded for chronic gastritis, active chronic gastritis, and density of H. pylori. Serum samples were analyzed for H. pylori by ELISA. The first set of assays for immunoglobulin G (IgG) and IgA used a pool of sonicated isolates of H. pylori from five patients in the United States (antigen A). The second set of assays, developed in the United Kingdom, used three different antigens: antigen 1, an acid-extractable surface antigen; antigen 2, an acid-extractable antigen from an aflagellate variant; and antigen 3, a urease-containing fraction. Cutoff scores for positive results were determined a priori on the basis of previous serological studies. There was close agreement between histology and culture. In the study population, 36{\%} of the individuals were H. pylori positive. The diagnostic value of the different ELISAs were highly comparable, and the crude antigens performed as well as the more purified antigens. The antigen A IgG had a sensitivity and specificity of 96 and 94{\%}, respectively; the values for antigen 1 were 93 and 96{\%}, respectively. The antigen A IgA and antigen 3 assays were the least sensitive tests. All of the serological assays were significantly associated with active chronic gastritis scores, but the IgA assay provided additional independent information for discriminating mild active gastritis from more severe gastritis. Despite the antigenic variation that exists between H. pylori strains, the diagnostic characteristics of these ELISAs from the United States and the United Kingdom are similarly highly sensitive and specific.",
author = "Talley, {N. J.} and Newell, {D. G.} and Ormand, {J. E.} and Carpenter, {H. A.} and Wilson, {W. R.} and Zinsmeister, {A. R.} and Perez-Perez, {G. I.} and Blaser, {M. J.}",
year = "1991",
language = "English (US)",
volume = "29",
pages = "1635--1639",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "8",

}

TY - JOUR

T1 - Serodiagnosis of Helicobacter pylori

T2 - Comparison of enzyme-linked immunosorbent assays

AU - Talley, N. J.

AU - Newell, D. G.

AU - Ormand, J. E.

AU - Carpenter, H. A.

AU - Wilson, W. R.

AU - Zinsmeister, A. R.

AU - Perez-Perez, G. I.

AU - Blaser, M. J.

PY - 1991

Y1 - 1991

N2 - Enzyme-linked immunosorbent assays (ELISAs) have been developed to diagnose Helicobacter pylori infection. However, the methods are not standardized. We therefore prospectively evaluated the sensitivities and specificities of ELISAs developed in the United States and the United Kingdom in a study population comprising 41 consecutive symptomatic outpatients and 35 volunteers. At endoscopy, multiple biopsies were obtained for histology and culture and stained sections were graded for chronic gastritis, active chronic gastritis, and density of H. pylori. Serum samples were analyzed for H. pylori by ELISA. The first set of assays for immunoglobulin G (IgG) and IgA used a pool of sonicated isolates of H. pylori from five patients in the United States (antigen A). The second set of assays, developed in the United Kingdom, used three different antigens: antigen 1, an acid-extractable surface antigen; antigen 2, an acid-extractable antigen from an aflagellate variant; and antigen 3, a urease-containing fraction. Cutoff scores for positive results were determined a priori on the basis of previous serological studies. There was close agreement between histology and culture. In the study population, 36% of the individuals were H. pylori positive. The diagnostic value of the different ELISAs were highly comparable, and the crude antigens performed as well as the more purified antigens. The antigen A IgG had a sensitivity and specificity of 96 and 94%, respectively; the values for antigen 1 were 93 and 96%, respectively. The antigen A IgA and antigen 3 assays were the least sensitive tests. All of the serological assays were significantly associated with active chronic gastritis scores, but the IgA assay provided additional independent information for discriminating mild active gastritis from more severe gastritis. Despite the antigenic variation that exists between H. pylori strains, the diagnostic characteristics of these ELISAs from the United States and the United Kingdom are similarly highly sensitive and specific.

AB - Enzyme-linked immunosorbent assays (ELISAs) have been developed to diagnose Helicobacter pylori infection. However, the methods are not standardized. We therefore prospectively evaluated the sensitivities and specificities of ELISAs developed in the United States and the United Kingdom in a study population comprising 41 consecutive symptomatic outpatients and 35 volunteers. At endoscopy, multiple biopsies were obtained for histology and culture and stained sections were graded for chronic gastritis, active chronic gastritis, and density of H. pylori. Serum samples were analyzed for H. pylori by ELISA. The first set of assays for immunoglobulin G (IgG) and IgA used a pool of sonicated isolates of H. pylori from five patients in the United States (antigen A). The second set of assays, developed in the United Kingdom, used three different antigens: antigen 1, an acid-extractable surface antigen; antigen 2, an acid-extractable antigen from an aflagellate variant; and antigen 3, a urease-containing fraction. Cutoff scores for positive results were determined a priori on the basis of previous serological studies. There was close agreement between histology and culture. In the study population, 36% of the individuals were H. pylori positive. The diagnostic value of the different ELISAs were highly comparable, and the crude antigens performed as well as the more purified antigens. The antigen A IgG had a sensitivity and specificity of 96 and 94%, respectively; the values for antigen 1 were 93 and 96%, respectively. The antigen A IgA and antigen 3 assays were the least sensitive tests. All of the serological assays were significantly associated with active chronic gastritis scores, but the IgA assay provided additional independent information for discriminating mild active gastritis from more severe gastritis. Despite the antigenic variation that exists between H. pylori strains, the diagnostic characteristics of these ELISAs from the United States and the United Kingdom are similarly highly sensitive and specific.

UR - http://www.scopus.com/inward/record.url?scp=0025816191&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025816191&partnerID=8YFLogxK

M3 - Article

C2 - 1761685

AN - SCOPUS:0025816191

VL - 29

SP - 1635

EP - 1639

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 8

ER -