Separation of plasmacytoid dendritic cells from B-cell-biased lymphoid progenitor (BLP) and Pre-pro B cells using PDCA-1

Kay L Medina, Sarah N. Tangen, Lauren M. Seaburg, Puspa Thapa, Kimberly A. Gwin, Virginia M Shapiro

Research output: Contribution to journalArticle

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Abstract

B-cell-biased lymphoid progenitors (BLPs) and Pre-pro B cells lie at a critical juncture between B cell specification and commitment. However, both of these populations are heterogenous, which hampers investigation into the molecular changes that occur as lymphoid progenitors commit to the B cell lineage. Here, we demonstrate that there are PDCA-1(+)Siglec H(+) plasmacytoid dendritic cells (pDCs) that co-purify with BLPs and Pre-pro B cells, which express little or no CD11c or Ly6C. Removal of PDCA-1(+) pDCs separates B cell progenitors that express high levels of a Rag1-GFP reporter from Rag1-GFP(low/neg) pDCs within the BLP and Pre-pro B populations. Analysis of Flt3-ligand knockout and IL-7Rα knockout mice revealed that there is a block in B cell development at the all-lymphoid progenitor (ALP) stage, as the majority of cells within the BLP or Pre-pro B gates were PDCA-1(+) pDCs. Thus, removal of PDCA-1(+) pDCs is critical for analysis of BLP and Pre-pro B cell populations. Analysis of B cell potential within the B220(+)CD19(-) fraction demonstrated that AA4.1(+)Ly6D(+)PDCA-1(-) Pre-pro B cells gave rise to CD19(+) B cells at high frequency, while PDCA-1(+) pDCs in this fraction did not. Interestingly, the presence of PDCA-1(+) pDCs within CLPs may help to explain the conflicting results regarding the origin of these cells.

Original languageEnglish (US)
Pages (from-to)e78408
JournalPLoS One
Volume8
Issue number10
DOIs
StatePublished - 2013

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B-Lymphoid Precursor Cells
dendritic cells
Dendritic Cells
B-lymphocytes
B-Lymphocytes
Cells
Sialic Acid Binding Ig-like Lectin 1
Population
Cell Lineage
Knockout Mice
Specifications

ASJC Scopus subject areas

  • Medicine(all)

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Separation of plasmacytoid dendritic cells from B-cell-biased lymphoid progenitor (BLP) and Pre-pro B cells using PDCA-1. / Medina, Kay L; Tangen, Sarah N.; Seaburg, Lauren M.; Thapa, Puspa; Gwin, Kimberly A.; Shapiro, Virginia M.

In: PLoS One, Vol. 8, No. 10, 2013, p. e78408.

Research output: Contribution to journalArticle

Medina, Kay L ; Tangen, Sarah N. ; Seaburg, Lauren M. ; Thapa, Puspa ; Gwin, Kimberly A. ; Shapiro, Virginia M. / Separation of plasmacytoid dendritic cells from B-cell-biased lymphoid progenitor (BLP) and Pre-pro B cells using PDCA-1. In: PLoS One. 2013 ; Vol. 8, No. 10. pp. e78408.
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