SDH and actomyosin ATPase activities of different fiber types in rat diaphragm muscle

G. C. Sieck, W. Z. Zhan, Y. S. Prakash, M. J. Daood, J. F. Watchko

Research output: Contribution to journalArticle

94 Scopus citations

Abstract

In the rat diaphragm muscle, the histochemical classification of type I, IIa, IIb, or IIx fibers was correlated with myosin heavy chain (MHC) immunoreactivity. Expression of MHC isoforms in single dissected fibers was also assessed electrophoretically. Most fibers (~86%) expressed a single MHC isoform, and when present, coexpression of MHC-2X and MHC-2B isoforms was most prevalent. Type I and IIa fibers were the smallest, type IIb fibers were the largest, and type IIx fibers were intermediate. Succinate dehydrogenase (SDH) and calcium-activated myosin adenosinetriphosphatase (actomyosin ATPase) activities were measured with quantitative histochemical procedures. Type I and IIa fibers had the highest SDH activities, followed in rank order by type IIx and IIb fibers. Type I fibers had the lowest actomyosin ATPase activity, followed in rank order by type IIa, IIx, and IIb fibers. Across all fibers, there was an inverse relationship between fiber SDH activity and cross-sectional area and a positive correlation between fiber actomyosin ATPase activity and cross-sectional area. The SDH and actomyosin ATPase activities of muscle fibers were also inversely correlated. These phenotypic differences in SDH and ATPase activities may be important in determining the contractile and fatigue properties of different fiber types in the rat diaphragm muscle.

Original languageEnglish (US)
Pages (from-to)1629-1639
Number of pages11
JournalJournal of applied physiology
Volume79
Issue number5
DOIs
StatePublished - Jan 1 1995

Keywords

  • adenosinetriphosphatase
  • histochemistry
  • immunohistochemistry
  • metabolic enzymes
  • morphometry
  • myosin heavy chain isoforms
  • succinate dehydrogenase

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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