Screening the human protocadherin 8 (PCDH8) gene in schizophrenia

N. J. Bray, G. Kirov, R. J. Owen, N. J. Jacobsen, L. Georgieva, H. J. Williams, N. Norton, G. Spurlock, S. Jones, S. Zammit, M. C. O'Donovan, M. J. Owen

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Abnormalities in synaptic connectivity and plasticity have been implicated in the pathophysiology of schizophrenia. Molecules involved in the development and maintenance of neural circuitry include the recently cloned protocadherins. Human protocadherin 8 (PCDH8) is homologous to 'arcadlin', a molecule shown to play a role in hippocampal synaptic function in the rat. The gene encoding PCDH8 maps to a region on chromosome 13 where linkage to schizophrenia has been reported. In this study, the entire expressed sequence of the PCDH8 gene and over 800bp of the 5′ flanking region were screened for polymorphisms in 30 DSM-IV schizophrenia individuals using Denaturing High Performance Liquid Chromatography (DHPLC). A total of nine single nucleotide polymorphisms were identified, including three in the first exon that are predicted to change the amino acid sequence. One polymorphism, causing the Trp7Arg change in the putative signal peptide, showed a trend towards excess of the arginine encoding allele in a case-control sample consisting of 520 DSM-IV schizophrenia patients and 535 matched controls from the UK (χ2 = 3.72, P [1df] = 0.054). However, this polymorphism did not show preferential transmission to schizophrenic individuals in a separate sample of 203 proband-parent trios from Bulgaria. A second, rare single nucleotide variation, predicting the non-conservative amino acid change Glu39Ala, was found in one schizophrenic individual and their affected sibling but not in a further 352 affected individuals, nor 357 controls. These results suggest that any contribution of PCDH8 polymorphisms to schizophrenia susceptibility is likely to be weak, although the existence of rare variations of stronger effect cannot be excluded.

Original languageEnglish (US)
Pages (from-to)187-191
Number of pages5
JournalGenes, Brain and Behavior
Volume1
Issue number3
DOIs
StatePublished - Aug 2002

Keywords

  • Association
  • Gene
  • Neurodevelopment
  • Polymorphism
  • Protocadherin
  • Schizophrenia

ASJC Scopus subject areas

  • Genetics
  • Neurology
  • Behavioral Neuroscience

Fingerprint Dive into the research topics of 'Screening the human protocadherin 8 (PCDH8) gene in schizophrenia'. Together they form a unique fingerprint.

  • Cite this

    Bray, N. J., Kirov, G., Owen, R. J., Jacobsen, N. J., Georgieva, L., Williams, H. J., Norton, N., Spurlock, G., Jones, S., Zammit, S., O'Donovan, M. C., & Owen, M. J. (2002). Screening the human protocadherin 8 (PCDH8) gene in schizophrenia. Genes, Brain and Behavior, 1(3), 187-191. https://doi.org/10.1034/j.1601-183X.2002.10307.x