Schwann cell‐conditioned medium supports neurite outgrowth and survival of spinal cord neurons in culture

E. Peter Bosch, Jose G. Assouline, Nicholas J. Pantazis, Ramon Lim Lim

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The effect of Schwann cell‐conditioned medium (SCM) on the development in vitro of spinal cord neurons was studied. Spinal cord neurons from 18‐day‐old rat embryos were cultured in serum‐free conditioned medium obtained from confluent rat Schwann cells. In cultures fed SCM, the cells developed typical neuronal morphology and were identified by indirect immunofluorescence using a monoclonal antibody to neurofilament protein. SCM stimulated neurite outgrowth and supported survival of spinal cord neurons. Preliminary characterization suggests that the neurotrophic factor in SCM appears to be a protein with a molecular weight greater than 8000 daltons.

Original languageEnglish (US)
Pages (from-to)324-330
Number of pages7
JournalMuscle & Nerve
Volume11
Issue number4
DOIs
StatePublished - Apr 1988

ASJC Scopus subject areas

  • Physiology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Physiology (medical)

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