RNAseq analysis of hippocampal microglia after kainic acid-induced seizures

Dale B. Bosco, Jiaying Zheng, Zhiyan Xu, Jiyun Peng, Ukpong B. Eyo, Ke Tang, Cheng Yan, Jun Huang, Lijie Feng, Gongxiong Wu, Jason R. Richardson, Hui Wang, Long Jun Wu

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Microglia have been shown to be of critical importance to the progression of temporal lobe epilepsy. However, the broad transcriptional changes that these cells undergo following seizure induction is not well understood. As such, we utilized RNAseq analysis upon microglia isolated from the hippocampus to determine expression pattern alterations following kainic acid induced seizure. We determined that microglia undergo dramatic changes to their expression patterns, particularly with regard to mitochondrial activity and metabolism. We also observed that microglia initiate immunological activity, specifically increasing interferon beta responsiveness. Our results provide novel insights into microglia transcriptional regulation following acute seizures and suggest potential therapeutic targets specifically in microglia for the treatment of seizures and epilepsy.

Original languageEnglish (US)
Article number34
JournalMolecular brain
Volume11
Issue number1
DOIs
StatePublished - Jun 20 2018

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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  • Cite this

    Bosco, D. B., Zheng, J., Xu, Z., Peng, J., Eyo, U. B., Tang, K., Yan, C., Huang, J., Feng, L., Wu, G., Richardson, J. R., Wang, H., & Wu, L. J. (2018). RNAseq analysis of hippocampal microglia after kainic acid-induced seizures. Molecular brain, 11(1), [34]. https://doi.org/10.1186/s13041-018-0376-5