Retromer maintains basolateral distribution of the type II TGF-β receptor via the recycling endosome

Xueqian Yin, Stephen J. Murphy, Mark C. Wilkes, Yan Ji, Edward B Leof

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Transforming growth factor β (TGF-β) is critical for the development and maintenance of epithelial structures. Because receptor localization and trafficking affect the cellular and organismal response to TGF-β, the present study was designed to address how such homeostatic control is regulated. To that end, we identify a new role for the mammalian retromer complex in maintaining basolateral plasma membrane expression of the type II TGF-β receptor (TβRII). Retromer and TβRII associate in the presence or absence of TGF-β ligand. After retromer knockdown, although TβRII internalization and trafficking to a Rab5-positive compartment occur as in wild-type cells, receptor recycling is inhibited. This results in TβRII mislocalization from the basolateral to both the basolateral and apical plasma membranes independent of Golgi transit and the Rab11-positive apical recycling endosome. The data support a model in which, after initial basolateral TβRII delivery, steady-state polarized TβRII expression is maintained by retromer/TβRII binding and delivery to the common recycling endosome.

Original languageEnglish (US)
Pages (from-to)2285-2298
Number of pages14
JournalMolecular Biology of the Cell
Volume24
Issue number14
DOIs
StatePublished - Jul 15 2013

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Growth Factor Receptors
Endosomes
Transforming Growth Factors
Cell Membrane
Maintenance
Ligands

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Retromer maintains basolateral distribution of the type II TGF-β receptor via the recycling endosome. / Yin, Xueqian; Murphy, Stephen J.; Wilkes, Mark C.; Ji, Yan; Leof, Edward B.

In: Molecular Biology of the Cell, Vol. 24, No. 14, 15.07.2013, p. 2285-2298.

Research output: Contribution to journalArticle

Yin, Xueqian ; Murphy, Stephen J. ; Wilkes, Mark C. ; Ji, Yan ; Leof, Edward B. / Retromer maintains basolateral distribution of the type II TGF-β receptor via the recycling endosome. In: Molecular Biology of the Cell. 2013 ; Vol. 24, No. 14. pp. 2285-2298.
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