TY - JOUR
T1 - Renal actions of synthetic Dendroaspis natriuretic peptide
AU - Lisy, Ondrej
AU - Jougasaki, Michihisa
AU - Heublein, Denise M.
AU - Schirger, John A.
AU - Chen, Horng H.
AU - Wennberg, Paul W.
AU - Burnett, John C.
N1 - Funding Information:
This research was supported by the grant from the National Kidney Foundation of Minnesota, Grant-in-Aid from the American Heart Association, Minnesota Affiliate-9806361X, Grant HL 36634 from the National Institutes of Health, the Miami Heart Research Institute, the Mayo Foundation, the Bruce and Ruth Rappaport Program in Vascular Biology, and the Robert and Irene Bestor and Stanley and Belle Bestor Funds. This work was presented as an abstract at the 71st Scientific Sessions of American Heart Association, November 8–11, 1998, Dallas, TX, USA ( Circulation 98:3920A, 1998). The first author received the 1998 Young Investigator Award from the Heart Failure Society of America for this investigation. The authors gratefully acknowledge the assistance of Ms. Gail Harty and Ms. Sharon S. Sandberg.
PY - 1999
Y1 - 1999
N2 - Background. Dendroaspis natriuretic peptide (DNP), recently isolated from the venom of the green Mamba snake Dendroaspis angusticeps, is a 38 amino acid peptide containing a 17 amino acid disulfide ring structure similar to that of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP). DNP-like immunoreactivity (DNP-LI) was reported to be present in human plasma and atrial myocardium and to be elevated in human congestive heart failure. Although previously named DNP, it remains unknown if DNP is natriuretic or if is it present in canine plasma, urine, and atrial myocardium. Method. Studies were performed in vivo in anesthetized dogs (N = 6) using intravenous infusion of synthetic DNP at 10 and 50 ng/kg/min. Employing a sensitive and specific radioimmunoassay for DNP, the presence of DNP-like peptide was assessed in the canine plasma and urine before, during, and following the administration of exogenous synthetic DNP. Additionally, we performed immunohistochemical studies using the indirect immunoperoxidase method with polyclonal DNP antiserum in normal atrial myocardium (N = 10). Atrial concentrations of DNP-LI were also assessed. Results. We report that DNP is markedly natriuretic and diuretic, which, like ANP and BNP, is associated with the increase in urinary and plasma cGMP. DNP-like peptide is also detected in canine plasma, urine, and atrial myocardium. Conclusion. These studies establish that DNP is a potent natriuretic and diuretic peptide with tubular actions linked to cGMP and that DNP may play a physiological role in the regulation of sodium excretion.
AB - Background. Dendroaspis natriuretic peptide (DNP), recently isolated from the venom of the green Mamba snake Dendroaspis angusticeps, is a 38 amino acid peptide containing a 17 amino acid disulfide ring structure similar to that of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP). DNP-like immunoreactivity (DNP-LI) was reported to be present in human plasma and atrial myocardium and to be elevated in human congestive heart failure. Although previously named DNP, it remains unknown if DNP is natriuretic or if is it present in canine plasma, urine, and atrial myocardium. Method. Studies were performed in vivo in anesthetized dogs (N = 6) using intravenous infusion of synthetic DNP at 10 and 50 ng/kg/min. Employing a sensitive and specific radioimmunoassay for DNP, the presence of DNP-like peptide was assessed in the canine plasma and urine before, during, and following the administration of exogenous synthetic DNP. Additionally, we performed immunohistochemical studies using the indirect immunoperoxidase method with polyclonal DNP antiserum in normal atrial myocardium (N = 10). Atrial concentrations of DNP-LI were also assessed. Results. We report that DNP is markedly natriuretic and diuretic, which, like ANP and BNP, is associated with the increase in urinary and plasma cGMP. DNP-like peptide is also detected in canine plasma, urine, and atrial myocardium. Conclusion. These studies establish that DNP is a potent natriuretic and diuretic peptide with tubular actions linked to cGMP and that DNP may play a physiological role in the regulation of sodium excretion.
KW - CGMP
KW - Dendroaspis angusticeps
KW - Myocardium
KW - Natriuretic hormones
KW - Sodium excretion
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U2 - 10.1046/j.1523-1755.1999.00573.x
DO - 10.1046/j.1523-1755.1999.00573.x
M3 - Article
C2 - 10432389
AN - SCOPUS:0032815463
SN - 0085-2538
VL - 56
SP - 502
EP - 508
JO - Kidney international
JF - Kidney international
IS - 2
ER -