Relationship between Microbiota of the Colonic Mucosa vs Feces and Symptoms, Colonic Transit, and Methane Production in Female Patients with Chronic Constipation

Gopanandan Parthasarathy, Jun Chen, Xianfeng Chen, Nicholas D Chia, Helen M. O'Connor, Patricia G. Wolf, H. Rex Gaskins, Adil Eddie Bharucha

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Background & Aims In fecal samples from patients with chronic constipation, the microbiota differs from that of healthy subjects. However, the profiles of fecal microbiota only partially replicate those of the mucosal microbiota. It is not clear whether these differences are caused by variations in diet or colonic transit, or are associated with methane production (measured by breath tests). We compared the colonic mucosal and fecal microbiota in patients with chronic constipation and in healthy subjects to investigate the relationships between microbiota and other parameters. Methods Sigmoid colonic mucosal and fecal microbiota samples were collected from 25 healthy women (controls) and 25 women with chronic constipation and evaluated by 16S ribosomal RNA gene sequencing (average, 49,186 reads/sample). We assessed associations between microbiota (overall composition and operational taxonomic units) and demographic variables, diet, constipation status, colonic transit, and methane production (measured in breath samples after oral lactulose intake). Results Fourteen patients with chronic constipation had slow colonic transit. The profile of the colonic mucosal microbiota differed between constipated patients and controls (P

Original languageEnglish (US)
Pages (from-to)367-379e1
JournalGastroenterology
Volume150
Issue number2
DOIs
StatePublished - Feb 1 2016

Fingerprint

Microbiota
Methane
Constipation
Feces
Mucous Membrane
Healthy Volunteers
16S Ribosomal RNA
Diet
RNA Sequence Analysis
Lactulose
Breath Tests
Sigmoid Colon
rRNA Genes
Demography

Keywords

  • Irritable Bowel Syndrome
  • Microbiome

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Relationship between Microbiota of the Colonic Mucosa vs Feces and Symptoms, Colonic Transit, and Methane Production in Female Patients with Chronic Constipation. / Parthasarathy, Gopanandan; Chen, Jun; Chen, Xianfeng; Chia, Nicholas D; O'Connor, Helen M.; Wolf, Patricia G.; Gaskins, H. Rex; Bharucha, Adil Eddie.

In: Gastroenterology, Vol. 150, No. 2, 01.02.2016, p. 367-379e1.

Research output: Contribution to journalArticle

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