Regulatory effect of cytokines on eosinophil degranulation

Takao Fujisawa, Randa Abu-Ghazaleh, Hirohito Kita, Colin J. Sanderson, Gerald J. Gleich

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Abstract

We tested the effects of different cytokines on IgA-and IgG-induced eosinophil degranulation in vitro to determine the potential interaction between eosinophils and mononuclear cells. Purified normodense eosinophils were incubated with cytokines (including rIL-1, rIL-2, rIL-3, rIL-4, rIL-5, rIL-6, IFN-γ, granulocyte-macrophage CSF stimulating factor (GM-CSF), and TNF) for 1 to 3 h after which Ig-coupled Sepharose 4B beads were added as targets and the mixtures were incubated with the eosinophils at 37°C for 4 h. The Ig used were secretory IgA (sIgA), serum IgA and IgG, and myeloma IgA and IgG. The release of eosinophil-derived neurotoxin (EDN) was measured by RIA as an index of degranulation. rIL-5 was the most potent enhancer of Ig-induced degranulation and increased EDN release by 48% for sIgA and 136% for IgG. The effect of rIL-5 appeared as quickly as 15 min after incubation of eosinophils, sIgA beads and IL-5. GM-CSF and rIL-3 also enhanced Ig-induced EDN release but less potently than rIL-5. GM-CSF and rIL-5 by themselves induced a small but significant release of EDN from eosinophils in the absence of Ig-coated beads; rIL-3 did not. However, IFN-γ suppressed sIgA-induced EDN release by 23%. The other cytokines did not have any effect on eosinophil degranulation. These results suggest that cytokines which induce eosinophil differentiation and proliferation during hematopoiesis also enhance the effector function of mature eosinophils and that IFN-γ partially down-regulates eosinophil degranulation.

Original languageEnglish (US)
Pages (from-to)642-646
Number of pages5
JournalJournal of Immunology
Volume144
Issue number2
StatePublished - Jan 15 1990

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Eosinophils
Eosinophil-Derived Neurotoxin
Cytokines
Secretory Immunoglobulin A
Immunoglobulin G
Granulocytes
Immunoglobulin A
Macrophages
Interleukin-5
Hematopoiesis
Sepharose
Down-Regulation
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

Fujisawa, T., Abu-Ghazaleh, R., Kita, H., Sanderson, C. J., & Gleich, G. J. (1990). Regulatory effect of cytokines on eosinophil degranulation. Journal of Immunology, 144(2), 642-646.

Regulatory effect of cytokines on eosinophil degranulation. / Fujisawa, Takao; Abu-Ghazaleh, Randa; Kita, Hirohito; Sanderson, Colin J.; Gleich, Gerald J.

In: Journal of Immunology, Vol. 144, No. 2, 15.01.1990, p. 642-646.

Research output: Contribution to journalArticle

Fujisawa, T, Abu-Ghazaleh, R, Kita, H, Sanderson, CJ & Gleich, GJ 1990, 'Regulatory effect of cytokines on eosinophil degranulation', Journal of Immunology, vol. 144, no. 2, pp. 642-646.
Fujisawa T, Abu-Ghazaleh R, Kita H, Sanderson CJ, Gleich GJ. Regulatory effect of cytokines on eosinophil degranulation. Journal of Immunology. 1990 Jan 15;144(2):642-646.
Fujisawa, Takao ; Abu-Ghazaleh, Randa ; Kita, Hirohito ; Sanderson, Colin J. ; Gleich, Gerald J. / Regulatory effect of cytokines on eosinophil degranulation. In: Journal of Immunology. 1990 ; Vol. 144, No. 2. pp. 642-646.
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