Regulation of cardiac CACNB2 by microRNA-499: Potential role in atrial fibrillation

Tian You Ling, Xiao Li Wang, Qiang Chai, Tong D Lu, John M. Stulak, Lyle D. Joyce, Richard C. Daly, Kevin L. Greason, Li Qun Wu, Win Kuang Shen, Yong-Mei Cha, Hon Chi Lee

Research output: Contribution to journalArticle

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Abstract

The L-type calcium channel (LTCC) is one of the major ion channels that are known to be associated with the electrical remodeling of atrial fibrillation (AF). In AF, there is significant downregulation of the LTCC, but the underlying mechanism for such downregulation is not clear. We have previously reported that microRNA-499 (miR-499) is significantly upregulated in patients with permanent AF and that KCNN3, the gene that encodes the small-conductance calcium-activated potassium channel 3 (SK3), is a target of miR-499. We found that CACNB2, an important subunit of the LTCC, is also a target of miR-499. We hypothesize that miR-499 plays an important role in AF electrical remodeling by regulating the expression of CACNB2 and the LTCC. In atrial tissue from patients with permanent AF, CACNB2 was significantly downregulated by 67% (n = 4, p < 0.05) compared to those from patients with no history of AF. Transfection of miR-499 mimic into HL-1 cells, a mouse hyperplastic atrial cardiac myocyte cell-line, resulted in the downregulation of CACNB2 protein expression, while that of miR-499 inhibitor upregulated CACNB2 protein expression. Binding of miR-499 to the 3′ untranslated region of CACNB2 was confirmed by luciferase reporter assay and by the increased presence of CACNB2 mRNA in Argonaute pulled-down microRNA-induced silencing complexes after transfection with the miR-499 mimic. In addition, downregulation of CACNB2 resulted in the downregulation of protein levels of the pore-forming α-subunit (CACNA1C). In conclusion, upregulation of atrial miR-499 induces the downregulation of CACNB2 expression and may contribute to the electrical remodeling in AF.

Original languageEnglish (US)
Pages (from-to)78-84
Number of pages7
JournalBBA Clinical
Volume7
DOIs
StatePublished - Jun 1 2017

Fingerprint

MicroRNAs
Atrial Fibrillation
Down-Regulation
L-Type Calcium Channels
Atrial Remodeling
Transfection
Small-Conductance Calcium-Activated Potassium Channels
Porins
3' Untranslated Regions
Luciferases
Ion Channels
Cardiac Myocytes
Proteins
Up-Regulation
Cell Line
Messenger RNA

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Medicine
  • Physiology (medical)

Cite this

Regulation of cardiac CACNB2 by microRNA-499 : Potential role in atrial fibrillation. / Ling, Tian You; Wang, Xiao Li; Chai, Qiang; Lu, Tong D; Stulak, John M.; Joyce, Lyle D.; Daly, Richard C.; Greason, Kevin L.; Wu, Li Qun; Shen, Win Kuang; Cha, Yong-Mei; Lee, Hon Chi.

In: BBA Clinical, Vol. 7, 01.06.2017, p. 78-84.

Research output: Contribution to journalArticle

Ling, TY, Wang, XL, Chai, Q, Lu, TD, Stulak, JM, Joyce, LD, Daly, RC, Greason, KL, Wu, LQ, Shen, WK, Cha, Y-M & Lee, HC 2017, 'Regulation of cardiac CACNB2 by microRNA-499: Potential role in atrial fibrillation', BBA Clinical, vol. 7, pp. 78-84. https://doi.org/10.1016/j.bbacli.2017.02.002
Ling, Tian You ; Wang, Xiao Li ; Chai, Qiang ; Lu, Tong D ; Stulak, John M. ; Joyce, Lyle D. ; Daly, Richard C. ; Greason, Kevin L. ; Wu, Li Qun ; Shen, Win Kuang ; Cha, Yong-Mei ; Lee, Hon Chi. / Regulation of cardiac CACNB2 by microRNA-499 : Potential role in atrial fibrillation. In: BBA Clinical. 2017 ; Vol. 7. pp. 78-84.
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abstract = "The L-type calcium channel (LTCC) is one of the major ion channels that are known to be associated with the electrical remodeling of atrial fibrillation (AF). In AF, there is significant downregulation of the LTCC, but the underlying mechanism for such downregulation is not clear. We have previously reported that microRNA-499 (miR-499) is significantly upregulated in patients with permanent AF and that KCNN3, the gene that encodes the small-conductance calcium-activated potassium channel 3 (SK3), is a target of miR-499. We found that CACNB2, an important subunit of the LTCC, is also a target of miR-499. We hypothesize that miR-499 plays an important role in AF electrical remodeling by regulating the expression of CACNB2 and the LTCC. In atrial tissue from patients with permanent AF, CACNB2 was significantly downregulated by 67{\%} (n = 4, p < 0.05) compared to those from patients with no history of AF. Transfection of miR-499 mimic into HL-1 cells, a mouse hyperplastic atrial cardiac myocyte cell-line, resulted in the downregulation of CACNB2 protein expression, while that of miR-499 inhibitor upregulated CACNB2 protein expression. Binding of miR-499 to the 3′ untranslated region of CACNB2 was confirmed by luciferase reporter assay and by the increased presence of CACNB2 mRNA in Argonaute pulled-down microRNA-induced silencing complexes after transfection with the miR-499 mimic. In addition, downregulation of CACNB2 resulted in the downregulation of protein levels of the pore-forming α-subunit (CACNA1C). In conclusion, upregulation of atrial miR-499 induces the downregulation of CACNB2 expression and may contribute to the electrical remodeling in AF.",
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T2 - Potential role in atrial fibrillation

AU - Ling, Tian You

AU - Wang, Xiao Li

AU - Chai, Qiang

AU - Lu, Tong D

AU - Stulak, John M.

AU - Joyce, Lyle D.

AU - Daly, Richard C.

AU - Greason, Kevin L.

AU - Wu, Li Qun

AU - Shen, Win Kuang

AU - Cha, Yong-Mei

AU - Lee, Hon Chi

PY - 2017/6/1

Y1 - 2017/6/1

N2 - The L-type calcium channel (LTCC) is one of the major ion channels that are known to be associated with the electrical remodeling of atrial fibrillation (AF). In AF, there is significant downregulation of the LTCC, but the underlying mechanism for such downregulation is not clear. We have previously reported that microRNA-499 (miR-499) is significantly upregulated in patients with permanent AF and that KCNN3, the gene that encodes the small-conductance calcium-activated potassium channel 3 (SK3), is a target of miR-499. We found that CACNB2, an important subunit of the LTCC, is also a target of miR-499. We hypothesize that miR-499 plays an important role in AF electrical remodeling by regulating the expression of CACNB2 and the LTCC. In atrial tissue from patients with permanent AF, CACNB2 was significantly downregulated by 67% (n = 4, p < 0.05) compared to those from patients with no history of AF. Transfection of miR-499 mimic into HL-1 cells, a mouse hyperplastic atrial cardiac myocyte cell-line, resulted in the downregulation of CACNB2 protein expression, while that of miR-499 inhibitor upregulated CACNB2 protein expression. Binding of miR-499 to the 3′ untranslated region of CACNB2 was confirmed by luciferase reporter assay and by the increased presence of CACNB2 mRNA in Argonaute pulled-down microRNA-induced silencing complexes after transfection with the miR-499 mimic. In addition, downregulation of CACNB2 resulted in the downregulation of protein levels of the pore-forming α-subunit (CACNA1C). In conclusion, upregulation of atrial miR-499 induces the downregulation of CACNB2 expression and may contribute to the electrical remodeling in AF.

AB - The L-type calcium channel (LTCC) is one of the major ion channels that are known to be associated with the electrical remodeling of atrial fibrillation (AF). In AF, there is significant downregulation of the LTCC, but the underlying mechanism for such downregulation is not clear. We have previously reported that microRNA-499 (miR-499) is significantly upregulated in patients with permanent AF and that KCNN3, the gene that encodes the small-conductance calcium-activated potassium channel 3 (SK3), is a target of miR-499. We found that CACNB2, an important subunit of the LTCC, is also a target of miR-499. We hypothesize that miR-499 plays an important role in AF electrical remodeling by regulating the expression of CACNB2 and the LTCC. In atrial tissue from patients with permanent AF, CACNB2 was significantly downregulated by 67% (n = 4, p < 0.05) compared to those from patients with no history of AF. Transfection of miR-499 mimic into HL-1 cells, a mouse hyperplastic atrial cardiac myocyte cell-line, resulted in the downregulation of CACNB2 protein expression, while that of miR-499 inhibitor upregulated CACNB2 protein expression. Binding of miR-499 to the 3′ untranslated region of CACNB2 was confirmed by luciferase reporter assay and by the increased presence of CACNB2 mRNA in Argonaute pulled-down microRNA-induced silencing complexes after transfection with the miR-499 mimic. In addition, downregulation of CACNB2 resulted in the downregulation of protein levels of the pore-forming α-subunit (CACNA1C). In conclusion, upregulation of atrial miR-499 induces the downregulation of CACNB2 expression and may contribute to the electrical remodeling in AF.

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