Regional variation in adipogenesis and IGF regulatory proteins in the fetal baboon

Yourka D. Tchoukalova, Peter W. Nathanielsz, Cheryl A Conover, Steven R. Smith, Eric Ravussin

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Intrauterine growth rate is associated with body distribution in adulthood suggesting differential response of fetal fat depots to nutritional modifications. We hypothesize that there is regional differences in fetal adipogenesis, in part, due to depot-specific regulation of the availability of insulin growth factors. In near-term baboon fetuses (n = 3-5), the subcutaneous abdominal vs. omental preadipocytes had (1) more extensive lipid accumulation as assessed by BODIPY (lipid staining) to DAPI (nuclei) absorbance ratios (mean ± SEM; 0.51 ± 0.21, 0.35 ± 0.09, p < 0.05), (2) lower (p < 0.05) secretion of IGF-binding protein 4 (9.6 ± 1.2 vs. 17.4 ± 2.8 ng/ml) and its protease pregnancy associated plasma protein A (24.6 ± 1.9 vs. 39.1 ± 6.3 μIU/ml), (3) lower protein expression of IGF2 "clearance" receptor in cell lysate (0.28 ± 0.03 vs. 0.53 ± 0.02 OD U/mm2, p < 0.05); all variables were intermediate in femoral preadipocytes. The regional variation of the adipogenesis and the IGF regulatory pathway set the stage for differential responsiveness of fat depots to external signals.

Original languageEnglish (US)
Pages (from-to)679-683
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume380
Issue number3
DOIs
StatePublished - Mar 13 2009

Fingerprint

Fetal Proteins
Adipogenesis
Papio
Fats
Insulin-Like Growth Factor Binding Protein 4
Pregnancy-Associated Plasma Protein-A
Lipids
Thigh
Intercellular Signaling Peptides and Proteins
Proteins
Fetus
Peptide Hydrolases
Availability
Insulin
Staining and Labeling
Scanning electron microscopy
Growth
4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
DAPI

Keywords

  • Adipogenesis
  • Adipose
  • Differentiation
  • Fat
  • Fetus
  • Insulin-like growth factor
  • Non-human primates
  • Preadipocyte
  • Pregnancy associated plasma protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Regional variation in adipogenesis and IGF regulatory proteins in the fetal baboon. / Tchoukalova, Yourka D.; Nathanielsz, Peter W.; Conover, Cheryl A; Smith, Steven R.; Ravussin, Eric.

In: Biochemical and Biophysical Research Communications, Vol. 380, No. 3, 13.03.2009, p. 679-683.

Research output: Contribution to journalArticle

Tchoukalova, Yourka D. ; Nathanielsz, Peter W. ; Conover, Cheryl A ; Smith, Steven R. ; Ravussin, Eric. / Regional variation in adipogenesis and IGF regulatory proteins in the fetal baboon. In: Biochemical and Biophysical Research Communications. 2009 ; Vol. 380, No. 3. pp. 679-683.
@article{92e6a9e4c83048209e753b1340f0aa60,
title = "Regional variation in adipogenesis and IGF regulatory proteins in the fetal baboon",
abstract = "Intrauterine growth rate is associated with body distribution in adulthood suggesting differential response of fetal fat depots to nutritional modifications. We hypothesize that there is regional differences in fetal adipogenesis, in part, due to depot-specific regulation of the availability of insulin growth factors. In near-term baboon fetuses (n = 3-5), the subcutaneous abdominal vs. omental preadipocytes had (1) more extensive lipid accumulation as assessed by BODIPY (lipid staining) to DAPI (nuclei) absorbance ratios (mean ± SEM; 0.51 ± 0.21, 0.35 ± 0.09, p < 0.05), (2) lower (p < 0.05) secretion of IGF-binding protein 4 (9.6 ± 1.2 vs. 17.4 ± 2.8 ng/ml) and its protease pregnancy associated plasma protein A (24.6 ± 1.9 vs. 39.1 ± 6.3 μIU/ml), (3) lower protein expression of IGF2 {"}clearance{"} receptor in cell lysate (0.28 ± 0.03 vs. 0.53 ± 0.02 OD U/mm2, p < 0.05); all variables were intermediate in femoral preadipocytes. The regional variation of the adipogenesis and the IGF regulatory pathway set the stage for differential responsiveness of fat depots to external signals.",
keywords = "Adipogenesis, Adipose, Differentiation, Fat, Fetus, Insulin-like growth factor, Non-human primates, Preadipocyte, Pregnancy associated plasma protein",
author = "Tchoukalova, {Yourka D.} and Nathanielsz, {Peter W.} and Conover, {Cheryl A} and Smith, {Steven R.} and Eric Ravussin",
year = "2009",
month = "3",
day = "13",
doi = "10.1016/j.bbrc.2009.01.149",
language = "English (US)",
volume = "380",
pages = "679--683",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Regional variation in adipogenesis and IGF regulatory proteins in the fetal baboon

AU - Tchoukalova, Yourka D.

AU - Nathanielsz, Peter W.

AU - Conover, Cheryl A

AU - Smith, Steven R.

AU - Ravussin, Eric

PY - 2009/3/13

Y1 - 2009/3/13

N2 - Intrauterine growth rate is associated with body distribution in adulthood suggesting differential response of fetal fat depots to nutritional modifications. We hypothesize that there is regional differences in fetal adipogenesis, in part, due to depot-specific regulation of the availability of insulin growth factors. In near-term baboon fetuses (n = 3-5), the subcutaneous abdominal vs. omental preadipocytes had (1) more extensive lipid accumulation as assessed by BODIPY (lipid staining) to DAPI (nuclei) absorbance ratios (mean ± SEM; 0.51 ± 0.21, 0.35 ± 0.09, p < 0.05), (2) lower (p < 0.05) secretion of IGF-binding protein 4 (9.6 ± 1.2 vs. 17.4 ± 2.8 ng/ml) and its protease pregnancy associated plasma protein A (24.6 ± 1.9 vs. 39.1 ± 6.3 μIU/ml), (3) lower protein expression of IGF2 "clearance" receptor in cell lysate (0.28 ± 0.03 vs. 0.53 ± 0.02 OD U/mm2, p < 0.05); all variables were intermediate in femoral preadipocytes. The regional variation of the adipogenesis and the IGF regulatory pathway set the stage for differential responsiveness of fat depots to external signals.

AB - Intrauterine growth rate is associated with body distribution in adulthood suggesting differential response of fetal fat depots to nutritional modifications. We hypothesize that there is regional differences in fetal adipogenesis, in part, due to depot-specific regulation of the availability of insulin growth factors. In near-term baboon fetuses (n = 3-5), the subcutaneous abdominal vs. omental preadipocytes had (1) more extensive lipid accumulation as assessed by BODIPY (lipid staining) to DAPI (nuclei) absorbance ratios (mean ± SEM; 0.51 ± 0.21, 0.35 ± 0.09, p < 0.05), (2) lower (p < 0.05) secretion of IGF-binding protein 4 (9.6 ± 1.2 vs. 17.4 ± 2.8 ng/ml) and its protease pregnancy associated plasma protein A (24.6 ± 1.9 vs. 39.1 ± 6.3 μIU/ml), (3) lower protein expression of IGF2 "clearance" receptor in cell lysate (0.28 ± 0.03 vs. 0.53 ± 0.02 OD U/mm2, p < 0.05); all variables were intermediate in femoral preadipocytes. The regional variation of the adipogenesis and the IGF regulatory pathway set the stage for differential responsiveness of fat depots to external signals.

KW - Adipogenesis

KW - Adipose

KW - Differentiation

KW - Fat

KW - Fetus

KW - Insulin-like growth factor

KW - Non-human primates

KW - Preadipocyte

KW - Pregnancy associated plasma protein

UR - http://www.scopus.com/inward/record.url?scp=60549104190&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=60549104190&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2009.01.149

DO - 10.1016/j.bbrc.2009.01.149

M3 - Article

C2 - 19285021

AN - SCOPUS:60549104190

VL - 380

SP - 679

EP - 683

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -