Regional differences in MRI detection of amyloid plaques in AD transgenic mouse brain

T. M. Wengenack, D. A. Reyes, G. L. Curran, B. J. Borowski, J. Lin, G. M. Preboske, S. S. Holasek, E. J. Gilles, R. Chamberlain, M. Marjanska, Clifford R Jr. Jack, M. Garwood, J. F. Poduslo

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Our laboratory and others have reported the ability to detect individual Alzheimer's disease (AD) amyloid plaques in transgenic mouse brain in vivo by magnetic resonance imaging (MRI). Since amyloid plaques contain iron, most MRI studies attempting to detect plaques in AD transgenic mouse brain have employed techniques that exploit the paramagnetic effect of iron and have had mixed results. In the present study, using five-way anatomic spatial coregistration of MR images with three different histological techniques, properties of amyloid plaques in AD transgenic mouse brain were revealed that may explain their variable visibility in gradient- and spin-echo MR images. The results demonstrate differences in the visibility of plaques in the cortex and hippocampus, compared to plaques in the thalamus, by the different MRI sequences. All plaques were equally detectable by T2SE, while only thalamic plaques were reliably detectable by T2*GE pulse sequences. Histology revealed that cortical/hippocampal plaques have low levels of iron while thalamic plaques have very high levels. However, the paramagnetic effect of iron does not appear to be the sole factor leading to the rapid decay of transverse magnetization (short T2) in cortical/hippocampal plaques. Accordingly, MRI methods that rely less on iron magnetic susceptibility effect may be more successful for eventual human AD plaque MR imaging, particularly since human AD plaques more closely resemble the cortical and hippocampal plaques of AD transgenic mice than thalamic plaques.

Original languageEnglish (US)
Pages (from-to)113-122
Number of pages10
JournalNeuroImage
Volume54
Issue number1
DOIs
StatePublished - Jan 1 2011

Fingerprint

Amyloid Plaques
Transgenic Mice
Alzheimer Disease
Magnetic Resonance Imaging
Iron
Brain
Histological Techniques
Aptitude
Thalamus
Hippocampus
Histology

Keywords

  • Alzheimer's disease
  • Amyloid-β
  • Iron
  • Magnetic resonance imaging
  • Thioflavine S

ASJC Scopus subject areas

  • Cognitive Neuroscience
  • Neurology

Cite this

Wengenack, T. M., Reyes, D. A., Curran, G. L., Borowski, B. J., Lin, J., Preboske, G. M., ... Poduslo, J. F. (2011). Regional differences in MRI detection of amyloid plaques in AD transgenic mouse brain. NeuroImage, 54(1), 113-122. https://doi.org/10.1016/j.neuroimage.2010.08.033

Regional differences in MRI detection of amyloid plaques in AD transgenic mouse brain. / Wengenack, T. M.; Reyes, D. A.; Curran, G. L.; Borowski, B. J.; Lin, J.; Preboske, G. M.; Holasek, S. S.; Gilles, E. J.; Chamberlain, R.; Marjanska, M.; Jack, Clifford R Jr.; Garwood, M.; Poduslo, J. F.

In: NeuroImage, Vol. 54, No. 1, 01.01.2011, p. 113-122.

Research output: Contribution to journalArticle

Wengenack, TM, Reyes, DA, Curran, GL, Borowski, BJ, Lin, J, Preboske, GM, Holasek, SS, Gilles, EJ, Chamberlain, R, Marjanska, M, Jack, CRJ, Garwood, M & Poduslo, JF 2011, 'Regional differences in MRI detection of amyloid plaques in AD transgenic mouse brain', NeuroImage, vol. 54, no. 1, pp. 113-122. https://doi.org/10.1016/j.neuroimage.2010.08.033
Wengenack TM, Reyes DA, Curran GL, Borowski BJ, Lin J, Preboske GM et al. Regional differences in MRI detection of amyloid plaques in AD transgenic mouse brain. NeuroImage. 2011 Jan 1;54(1):113-122. https://doi.org/10.1016/j.neuroimage.2010.08.033
Wengenack, T. M. ; Reyes, D. A. ; Curran, G. L. ; Borowski, B. J. ; Lin, J. ; Preboske, G. M. ; Holasek, S. S. ; Gilles, E. J. ; Chamberlain, R. ; Marjanska, M. ; Jack, Clifford R Jr. ; Garwood, M. ; Poduslo, J. F. / Regional differences in MRI detection of amyloid plaques in AD transgenic mouse brain. In: NeuroImage. 2011 ; Vol. 54, No. 1. pp. 113-122.
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