TY - JOUR
T1 - Regional and cellular distribution of DREAM in adult rat brain consistent with multiple sensory processing roles
AU - Hammond, Pamela I.
AU - Craig, Theodore A.
AU - Kumar, Rajiv
AU - Brimijoin, Stephen
N1 - Funding Information:
This research was supported by NIH Grants DK25409, DK58546, AR27032 (to RK). The authors wish to acknowledge Dr. E. Holmuhamedov for the design of the digital camera-to-microscope interface which permitted high magnification–high resolution digital photomicrography.
PY - 2003/3/17
Y1 - 2003/3/17
N2 - We used immunohistochemistry to map the recently discovered EF-hand protein, DREAM (downstream regulatory element antagonist modulator), and compare its expression in rat brain with that of other key neural proteins. DREAM immunoreactivity was most intense in cerebellar granular cortex. That expression pattern matches one reported for Kv4.2, an interaction partner of DREAM in regulation of potassium channels. On the other hand, the regional and cellular expression of DREAM in cerebellum was opposite to that of calbindin D28k. Other loci for DREAM expression included the hippocampus and retrosplenial granular cortex, which share afferent and efferent connections. A notable trend, however, was the consistent appearance of DREAM at primary sites for sensory processing. These included the optic tract, superior colliculus, olfactory bulb, and several thalamic relay centers such as the anterior dorsal, medial geniculate, dorsolateral geniculate, ventral posteromedial and ventral posterolateral nuclei. Altogether, the results are consistent with multiple functions for DREAM, including a potential role in transfer of sensory information.
AB - We used immunohistochemistry to map the recently discovered EF-hand protein, DREAM (downstream regulatory element antagonist modulator), and compare its expression in rat brain with that of other key neural proteins. DREAM immunoreactivity was most intense in cerebellar granular cortex. That expression pattern matches one reported for Kv4.2, an interaction partner of DREAM in regulation of potassium channels. On the other hand, the regional and cellular expression of DREAM in cerebellum was opposite to that of calbindin D28k. Other loci for DREAM expression included the hippocampus and retrosplenial granular cortex, which share afferent and efferent connections. A notable trend, however, was the consistent appearance of DREAM at primary sites for sensory processing. These included the optic tract, superior colliculus, olfactory bulb, and several thalamic relay centers such as the anterior dorsal, medial geniculate, dorsolateral geniculate, ventral posteromedial and ventral posterolateral nuclei. Altogether, the results are consistent with multiple functions for DREAM, including a potential role in transfer of sensory information.
KW - Brain
KW - Calsenilin
KW - Cerebellum
KW - DREAM
KW - Immunohistochemistry
KW - KChIP3
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U2 - 10.1016/S0169-328X(02)00693-9
DO - 10.1016/S0169-328X(02)00693-9
M3 - Article
C2 - 12654510
AN - SCOPUS:0346665903
SN - 0169-328X
VL - 111
SP - 104
EP - 110
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1-2
ER -